Tissue protein and lipid alterations in response to metallic impaction

Shari A. Seidman, Natasha A. Johnson, Ulises Arbelo, Katyayini Aribindi, Sanjoy K Bhattacharya

Research output: Contribution to journalArticle

Abstract

Tissue response to metallic contact and injury remains poorly understood at the level of proteins and lipid changes. We used corneas as a model system (bovine, porcine [n = 300 each], and human corneas [n = 6]) to characterize proteomic and lipidomic reactions to metallic exposure (impaction with iron). We also made a limited investigation into protein extractability and profile changes due to copper, and lead exposure as well. We identified selected proteins after trypsin digestion using an LCQ Deca XP and lipids using a TSQ Quantum Access Max mass spectrometer, respectively. Our findings indicate that iron impaction to corneal tissue results in cleavage of 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-2 variant (PLCB2; 134 kDa) into a 36 kDa species and presence of the epithelial layer is necessary for this cleavage. Penetration of the cornea with other metals (copper and lead) resulted in lower protein extractability from corneal tissue compared with controls but not distinct changes in PLCB2. The changes in protein profiles were unique to the type of metal used for impaction. The depth of injury negatively affected protein extractability and profile compared with controls. These changes were also dependent on several other factors in a complex manner. Iron impaction of corneal tissue for 24 hours results in cleavage of PLCB2 commensurate with significant changes in phosphatidylinositols but not phosphatidylcholines or other phospholipids.

Original languageEnglish (US)
JournalJournal of Cellular Biochemistry
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Tissue
Lipids
Cornea
Proteins
Iron
Copper
Metals
Phosphoinositide Phospholipase C
Wounds and Injuries
Mass spectrometers
Phosphatidylinositols
Phosphatidylcholines
Proteomics
Trypsin
Digestion
Phospholipids
Swine

Keywords

  • cell biology
  • cornea
  • HPLC
  • immunohistochemistry
  • iron
  • mass spectrometry
  • metallic impaction
  • protein identification
  • protein sequence

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Tissue protein and lipid alterations in response to metallic impaction. / Seidman, Shari A.; Johnson, Natasha A.; Arbelo, Ulises; Aribindi, Katyayini; Bhattacharya, Sanjoy K.

In: Journal of Cellular Biochemistry, 01.01.2018.

Research output: Contribution to journalArticle

Seidman, Shari A. ; Johnson, Natasha A. ; Arbelo, Ulises ; Aribindi, Katyayini ; Bhattacharya, Sanjoy K. / Tissue protein and lipid alterations in response to metallic impaction. In: Journal of Cellular Biochemistry. 2018.
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