Tissue engineering of functional salivary gland tissue

Akira Joraku, Christopher A. Sullivan, James J. Yoo, Anthony Atala

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Objectives: Treatment of conditions that lead to salivary hypofunction, such as radiation induced xerostomia, is currently limited to the administration of saliva substitutes and sialogogues. The transient effect of these medications necessitates frequent administration, and systemic side effects may be intolerable. The creation of implantable, functional salivary gland tissue from autologous glandular cells would provide a physiologic solution to this problem. We investigated the feasibility of engineering such tissue in vivo. Methods: Primary human salivary gland cells were grown, expanded, and seeded on biodegradable polymer scaffolds. A total of 48 scaffolds with cells and 16 control polymers without cells were implanted subcutaneously in athymic mice. The implants were retrieved 2, 4, and 8 weeks after the implantation for phenotypic and functional analyses. Results: Human salivary gland epithelial cells retained their phenotypic and functional characteristics at all culture stages. Histologically, formation of acinar gland-like structures was observed within the engineered tissue by 4 weeks after implantation. Immunocytochemical and Western blot analyses of the implanted tissues demonstrated the expression of human a-amylase, cytokeratins AE1/ AE3, and aqusporin-5 using cell-specific antibodies. Reverse-transcription polymerase chain reaction analyses confirmed the expression of human salivary type of α-amylase (s-amylase) mRNA. The retrieved tissues demonstrated the production of human α-amylase over time using a biochemical amylase detection system.

Original languageEnglish
Pages (from-to)244-248
Number of pages5
JournalLaryngoscope
Volume115
Issue number2
DOIs
StatePublished - Feb 1 2005
Externally publishedYes

Fingerprint

Tissue Engineering
Salivary Glands
Amylases
Polymers
Xerostomia
Keratins
Saliva
Nude Mice
Reverse Transcription
Western Blotting
Epithelial Cells
Radiation
Polymerase Chain Reaction
Messenger RNA
Antibodies

Keywords

  • Salivary glands
  • Tissue engineering

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Joraku, A., Sullivan, C. A., Yoo, J. J., & Atala, A. (2005). Tissue engineering of functional salivary gland tissue. Laryngoscope, 115(2), 244-248. https://doi.org/10.1097/01.mlg.0000154726.77915.cc

Tissue engineering of functional salivary gland tissue. / Joraku, Akira; Sullivan, Christopher A.; Yoo, James J.; Atala, Anthony.

In: Laryngoscope, Vol. 115, No. 2, 01.02.2005, p. 244-248.

Research output: Contribution to journalArticle

Joraku, A, Sullivan, CA, Yoo, JJ & Atala, A 2005, 'Tissue engineering of functional salivary gland tissue', Laryngoscope, vol. 115, no. 2, pp. 244-248. https://doi.org/10.1097/01.mlg.0000154726.77915.cc
Joraku, Akira ; Sullivan, Christopher A. ; Yoo, James J. ; Atala, Anthony. / Tissue engineering of functional salivary gland tissue. In: Laryngoscope. 2005 ; Vol. 115, No. 2. pp. 244-248.
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