Tissue engineering a complete vaginal replacement from a small biopsy of autologous tissue

Roger E. De Philippo, Colin E. Bishop, Luiz Freitas Filho, James J. Yoo, Anthony Atala

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

BACKGROUND.: In women, a healthy, patent vagina is important for the maintenance of a good quality of life. Apart from congenital abnormalities, such as cloacal exstrophy, intersex disorders, and an absence of the posterior two thirds of the organ, individuals may also suffer from cancer, trauma, infection, inflammation, or iatrogenic injuries leading to tissue damage and loss-all of which require vaginal repair or replacement. Of necessity, reconstruction is often performed with nonvaginal tissue substitutes, such as segments of large intestine or skin, which are not anatomically or functionally ideal (Hendren and Atala, J Urol 1994; 152: 752; Hendren and Atala, J Pediatr Surg 1995; 30: 91). Whenever such tissue is used additional complications often ensue, such as strictures, infection, hair growth, graft shrinkage, diverticuli, and even malignancy (Filipas et al., BJU Int 2000; 85: 715; Lai and Chang, Changgeng Yi Xue Za Zhi 1999; 22: 253; Parsons et al., J Pediatr Surg 2002; 37: 629; Seccia et al., Ann Plast Surg 2002; 49: 379; Filipas, Curr Opin Urol 2001; 11: 267). METHODS.: Using a rabbit model, we report here the construction of a functional vagina using autologous cells expanded from a small vaginal biopsy. RESULTS.: Six months after total vaginal replacement, radiographic analysis of rabbits implanted with the neovagina demonstrated wide, patent vaginal calibers without strictures. Histologic analysis revealed well-organized epithelial and muscle cell layers. Physiologic studies showed normal-range responses to electrical stimulation or to an adrenergic agonist. CONCLUSIONS.: These data indicate that a tissue engineering approach to clinical vaginal reconstruction in women is now a realistic possibility.

Original languageEnglish
Pages (from-to)208-214
Number of pages7
JournalTransplantation
Volume86
Issue number2
DOIs
StatePublished - Jul 27 2008
Externally publishedYes

Fingerprint

Tissue Engineering
Vagina
Biopsy
Pathologic Constriction
Rabbits
Adrenergic Agonists
Large Intestine
Wounds and Injuries
Infection
Hair
Muscle Cells
Electric Stimulation
Neoplasms
Reference Values
Epithelial Cells
Quality of Life
Inflammation
Transplants
Skin
Growth

Keywords

  • Regenerative medicine
  • Replacement organs
  • Tissue engineering
  • Vagina

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

De Philippo, R. E., Bishop, C. E., Filho, L. F., Yoo, J. J., & Atala, A. (2008). Tissue engineering a complete vaginal replacement from a small biopsy of autologous tissue. Transplantation, 86(2), 208-214. https://doi.org/10.1097/TP.0b013e31817f1686

Tissue engineering a complete vaginal replacement from a small biopsy of autologous tissue. / De Philippo, Roger E.; Bishop, Colin E.; Filho, Luiz Freitas; Yoo, James J.; Atala, Anthony.

In: Transplantation, Vol. 86, No. 2, 27.07.2008, p. 208-214.

Research output: Contribution to journalArticle

De Philippo, RE, Bishop, CE, Filho, LF, Yoo, JJ & Atala, A 2008, 'Tissue engineering a complete vaginal replacement from a small biopsy of autologous tissue', Transplantation, vol. 86, no. 2, pp. 208-214. https://doi.org/10.1097/TP.0b013e31817f1686
De Philippo, Roger E. ; Bishop, Colin E. ; Filho, Luiz Freitas ; Yoo, James J. ; Atala, Anthony. / Tissue engineering a complete vaginal replacement from a small biopsy of autologous tissue. In: Transplantation. 2008 ; Vol. 86, No. 2. pp. 208-214.
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