TY - JOUR
T1 - Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion
AU - Bodin, Aase
AU - Bharadwaj, Shantaram
AU - Wu, Shaofeng
AU - Gatenholm, Paul
AU - Atala, Anthony
AU - Zhang, Yuanyuan
N1 - Funding Information:
This work was funded by WFIRM and the Swedish Governmental Agency for Innovation Systems (VINNOVA) , who is greatly acknowledged. The authors AB and PG would also like to thank Arterion AB for making this study possible. Moreover does AB express gratitude to the BBV project at Chalmers for providing lab space. Finally, the authors would like to thank Dr. Jennifer Olson and Ms. Karen Klein for their editorial assistance.
PY - 2010/12
Y1 - 2010/12
N2 - The objective of this study was to generate bacterial cellulose (BC) scaffolds seeded with human urine-derived stem cells (USC) to form a tissue-engineered conduit for use in urinary diversion. Microporous BC scaffolds were synthesized and USC were induced to differentiate into urothelial and smooth muscle cells (SMC). Induced USC (106 cells/cm2) were seeded onto BC under static and 3D dynamic (10 or 40 RPM) conditions and cultured for 2 weeks. The urothelial cells and SMC derived from USC formed multilayers on the BC scaffold surface, and some cells infiltrated into the scaffold. The urothelium derived from USC differentiation expressed urothelial markers (uroplakin Ia and AE1/AE3) and the SMC expressed SMC markers (α-smooth muscle actin and desmin). In addition, USC/BC scaffold constructs were implanted into athymic mice, and the cells were tracked using immunohistochemical staining for human nuclear antigen. In vivo, the cells appeared to differentiate and express urothelial and SMC markers. In conclusion, porous BC scaffolds allow 3 dimensional growth of USC, leading to formation of a multilayered urothelium and cell-matrix infiltration. Thus, cell-seeded BC scaffolds hold promise for use in tissue-engineered urinary conduits for urinary reconstruction.
AB - The objective of this study was to generate bacterial cellulose (BC) scaffolds seeded with human urine-derived stem cells (USC) to form a tissue-engineered conduit for use in urinary diversion. Microporous BC scaffolds were synthesized and USC were induced to differentiate into urothelial and smooth muscle cells (SMC). Induced USC (106 cells/cm2) were seeded onto BC under static and 3D dynamic (10 or 40 RPM) conditions and cultured for 2 weeks. The urothelial cells and SMC derived from USC formed multilayers on the BC scaffold surface, and some cells infiltrated into the scaffold. The urothelium derived from USC differentiation expressed urothelial markers (uroplakin Ia and AE1/AE3) and the SMC expressed SMC markers (α-smooth muscle actin and desmin). In addition, USC/BC scaffold constructs were implanted into athymic mice, and the cells were tracked using immunohistochemical staining for human nuclear antigen. In vivo, the cells appeared to differentiate and express urothelial and SMC markers. In conclusion, porous BC scaffolds allow 3 dimensional growth of USC, leading to formation of a multilayered urothelium and cell-matrix infiltration. Thus, cell-seeded BC scaffolds hold promise for use in tissue-engineered urinary conduits for urinary reconstruction.
KW - Cell-matrix infiltration
KW - Cellulose
KW - Mesenchcymal stem cells
KW - Tissue-engineered urinary conduit
KW - Urinary tract
UR - http://www.scopus.com/inward/record.url?scp=77957575140&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957575140&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2010.07.108
DO - 10.1016/j.biomaterials.2010.07.108
M3 - Article
C2 - 20800278
AN - SCOPUS:77957575140
VL - 31
SP - 8889
EP - 8901
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 34
ER -