Tissue-engineered autologous vaginal organs in patients: A pilot cohort study

Atlántida M. Raya-Rivera, Diego Esquiliano, Reyna Fierro-Pastrana, Esther López-Bayghen, Pedro Valencia, Ricardo Ordorica-Flores, Shay Soker, James J. Yoo, Anthony Atala

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Background Several disorders might require vaginal reconstruction, such as congenital abnormalities, injury, or cancer. Reconstructive techniques for which non-vaginal tissue is used can be associated with complications. We assessed the use of engineered vaginal organs in four patients with vaginal aplasia caused by Mayer-Rokitansky-Küster-Hauser syndrome (MRKHS). Methods We invited to participate four consecutive patients who presented over a 3-year period with congenital vaginal aplasia due to MRKHS. Patients were aged 13-18 years. We obtained a vulvar biopsy of autologous tissue from every patient. We cultured, expanded, and seeded epithelial and muscle cells onto biodegradable scaff olds. The organs were constructed and allowed to mature in an incubator in a facility approved for human-tissue manufacturing. We used a perineal approach to surgically implant these organs. We recorded history, physical examination, vaginoscopy, serial tissue biopsies, MRIs, and self-administered Female Sexual Function Index questionnaire results for all patients, with a follow-up of up to 8 years. Findings We noted no long-term postoperative surgical complications. Yearly serial biopsies showed a tri-layered structure, consisting of an epithelial cell-lined lumen surrounded by matrix and muscle, with expected components of vaginal tissue present. Immunohistochemical analysis confi rmed the presence of phenotypically normal smooth muscle and epithelia. The MRIs, which showed the extent of the vaginal aplasia before surgery, showed the engineered organs and the absence of abnormalities after surgery, which was confi rmed with yearly vaginoscopy. A validated selfadministered Female Sexual Function Index questionnaire showed variables in the normal range in all areas tested, such as desire, arousal, lubrication, orgasm, satisfaction, and painless intercourse. Interpretation Vaginal organs, engineered from the patient's own cells and implanted, showed normal structural and functional variables with a follow-up of up to 8 years. These technologies could be useful in patients requiring vaginal reconstruction. Funding Wake Forest University and Hospital Infantil de México Federico Gómez.

Original languageEnglish
Pages (from-to)329-336
Number of pages8
JournalThe Lancet
Volume384
Issue number9940
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

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Cohort Studies
Biopsy
Epithelial Cells
Orgasm
Lubrication
Incubators
Arousal
Muscle Cells
Physical Examination
Smooth Muscle
Reference Values
Epithelium
History
Technology
Muscles
Wounds and Injuries
Neoplasms
Surveys and Questionnaires

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Raya-Rivera, A. M., Esquiliano, D., Fierro-Pastrana, R., López-Bayghen, E., Valencia, P., Ordorica-Flores, R., ... Atala, A. (2014). Tissue-engineered autologous vaginal organs in patients: A pilot cohort study. The Lancet, 384(9940), 329-336. https://doi.org/10.1016/S0140-6736(14)60542-0

Tissue-engineered autologous vaginal organs in patients : A pilot cohort study. / Raya-Rivera, Atlántida M.; Esquiliano, Diego; Fierro-Pastrana, Reyna; López-Bayghen, Esther; Valencia, Pedro; Ordorica-Flores, Ricardo; Soker, Shay; Yoo, James J.; Atala, Anthony.

In: The Lancet, Vol. 384, No. 9940, 01.01.2014, p. 329-336.

Research output: Contribution to journalArticle

Raya-Rivera, AM, Esquiliano, D, Fierro-Pastrana, R, López-Bayghen, E, Valencia, P, Ordorica-Flores, R, Soker, S, Yoo, JJ & Atala, A 2014, 'Tissue-engineered autologous vaginal organs in patients: A pilot cohort study', The Lancet, vol. 384, no. 9940, pp. 329-336. https://doi.org/10.1016/S0140-6736(14)60542-0
Raya-Rivera AM, Esquiliano D, Fierro-Pastrana R, López-Bayghen E, Valencia P, Ordorica-Flores R et al. Tissue-engineered autologous vaginal organs in patients: A pilot cohort study. The Lancet. 2014 Jan 1;384(9940):329-336. https://doi.org/10.1016/S0140-6736(14)60542-0
Raya-Rivera, Atlántida M. ; Esquiliano, Diego ; Fierro-Pastrana, Reyna ; López-Bayghen, Esther ; Valencia, Pedro ; Ordorica-Flores, Ricardo ; Soker, Shay ; Yoo, James J. ; Atala, Anthony. / Tissue-engineered autologous vaginal organs in patients : A pilot cohort study. In: The Lancet. 2014 ; Vol. 384, No. 9940. pp. 329-336.
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AU - Fierro-Pastrana, Reyna

AU - López-Bayghen, Esther

AU - Valencia, Pedro

AU - Ordorica-Flores, Ricardo

AU - Soker, Shay

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N2 - Background Several disorders might require vaginal reconstruction, such as congenital abnormalities, injury, or cancer. Reconstructive techniques for which non-vaginal tissue is used can be associated with complications. We assessed the use of engineered vaginal organs in four patients with vaginal aplasia caused by Mayer-Rokitansky-Küster-Hauser syndrome (MRKHS). Methods We invited to participate four consecutive patients who presented over a 3-year period with congenital vaginal aplasia due to MRKHS. Patients were aged 13-18 years. We obtained a vulvar biopsy of autologous tissue from every patient. We cultured, expanded, and seeded epithelial and muscle cells onto biodegradable scaff olds. The organs were constructed and allowed to mature in an incubator in a facility approved for human-tissue manufacturing. We used a perineal approach to surgically implant these organs. We recorded history, physical examination, vaginoscopy, serial tissue biopsies, MRIs, and self-administered Female Sexual Function Index questionnaire results for all patients, with a follow-up of up to 8 years. Findings We noted no long-term postoperative surgical complications. Yearly serial biopsies showed a tri-layered structure, consisting of an epithelial cell-lined lumen surrounded by matrix and muscle, with expected components of vaginal tissue present. Immunohistochemical analysis confi rmed the presence of phenotypically normal smooth muscle and epithelia. The MRIs, which showed the extent of the vaginal aplasia before surgery, showed the engineered organs and the absence of abnormalities after surgery, which was confi rmed with yearly vaginoscopy. A validated selfadministered Female Sexual Function Index questionnaire showed variables in the normal range in all areas tested, such as desire, arousal, lubrication, orgasm, satisfaction, and painless intercourse. Interpretation Vaginal organs, engineered from the patient's own cells and implanted, showed normal structural and functional variables with a follow-up of up to 8 years. These technologies could be useful in patients requiring vaginal reconstruction. Funding Wake Forest University and Hospital Infantil de México Federico Gómez.

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