Tissue and tumor expression of a cell surface glycoprotein complex containing an integral membrane glycoprotein activator of p185neu

Kai Wu, Pedro J Salas, Lisa Yee, Nevis L. Fregien, Kermit L. Carraway

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Ascites 13762 rat mammary adenocarcinoma cells contain an abundant heterodimeric cell surface glycoprotein complex. It is composed of a transmembrane subunit and a sialomucin subunit and is the product of a single gene. The transmembrane subunit has two EGF-like domains and activates the proto-oncogene receptor kinase p185neu. Southern blot comparisons of the ascites tumor and rat liver demonstrated the presence of the gene encoding the complex in normal tissues and showed an amplification of about fivefold in the ascites tumor. Polymerase chain reaction assays showed the presence of mRNA for the complex in rat brain and lung, but not in liver, pancreas, placenta, intestine, kidney, ovary and uterus. Northern blot analyses showed that the 9 kb transcript for the complex is expressed at an approximately 500-fold higher level in the ascites cells than in rat brain. Immunocytochemical studies using antiserum directed against the transmembrane subunit showed its presence in bronchial epithelium, brain ependymal and neurons of four day old animals and in the endoderm and neuronal cells of embryos. Similar immunocytochemical studies showed the presence of the transmembrane subunit in some human breast tumors. These results suggest that the gene encoding this complex is regulated in a tissue-specific manner, is overexpressed in some tumors and may play a role in tumor progesssion.

Original languageEnglish
Pages (from-to)3139-3147
Number of pages9
JournalOncogene
Volume9
Issue number11
StatePublished - Nov 1 1994

Fingerprint

Membrane Glycoproteins
Ascites
Neoplasms
Brain
Sialomucins
Genes
Endoderm
Proto-Oncogenes
Liver
Southern Blotting
Epidermal Growth Factor
Northern Blotting
Placenta
Uterus
Intestines
Immune Sera
Pancreas
Ovary
Adenocarcinoma
Breast

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Tissue and tumor expression of a cell surface glycoprotein complex containing an integral membrane glycoprotein activator of p185neu. / Wu, Kai; Salas, Pedro J; Yee, Lisa; Fregien, Nevis L.; Carraway, Kermit L.

In: Oncogene, Vol. 9, No. 11, 01.11.1994, p. 3139-3147.

Research output: Contribution to journalArticle

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