Timing of human sperm chromosome replication following fertilization of hamster eggs in vitro

Wayne Balkan; Renée H. Martin

doi:10.1002/mrd.1120060204

Timing of human sperm chromosome replication following fertilization of hamster eggs in vitro

Wayne Balkan, Renée H. Martin

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The visualization of human male pronuclear chromosomes is possible by utilizing a technique in which human sperm fertilize zona‐free Golden hamster (Mesocricetus auratus) ova in vitro. R banding of these chromosomes can be achieved by adding 20 μg/ml 5‐bromode‐oxyuridine (BrdU), a thymidine analogue, to the culture medium during mid‐to‐late S phase and subsequently staining the chromosomes with 0.1 mg/ml acridine orange. BrdU was added 3.0–10.0 hr postinsemination (hr pi). R bands were obtained when fertilized eggs were transferred into medium containing BrdU between 5.0 and 6.5 hr pi. This indicates that the human (male) pronuclear chromosomes were in mid‐to‐late S phase at these times.

Original language	English (US)
Pages (from-to)	115-119
Number of pages	5
Journal	Gamete Research
Volume	6
Issue number	2
DOIs	https://doi.org/10.1002/mrd.1120060204
State	Published - 1982
Externally published	Yes

Keywords

DNA synthesis
R banding
cross‐species fertilization
human sperm chromosomes

ASJC Scopus subject areas

Genetics
Developmental Biology

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title = "Timing of human sperm chromosome replication following fertilization of hamster eggs in vitro",

abstract = "The visualization of human male pronuclear chromosomes is possible by utilizing a technique in which human sperm fertilize zona‐free Golden hamster (Mesocricetus auratus) ova in vitro. R banding of these chromosomes can be achieved by adding 20 μg/ml 5‐bromode‐oxyuridine (BrdU), a thymidine analogue, to the culture medium during mid‐to‐late S phase and subsequently staining the chromosomes with 0.1 mg/ml acridine orange. BrdU was added 3.0–10.0 hr postinsemination (hr pi). R bands were obtained when fertilized eggs were transferred into medium containing BrdU between 5.0 and 6.5 hr pi. This indicates that the human (male) pronuclear chromosomes were in mid‐to‐late S phase at these times.",

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AU - Balkan, Wayne

AU - Martin, Renée H.

PY - 1982

Y1 - 1982

N2 - The visualization of human male pronuclear chromosomes is possible by utilizing a technique in which human sperm fertilize zona‐free Golden hamster (Mesocricetus auratus) ova in vitro. R banding of these chromosomes can be achieved by adding 20 μg/ml 5‐bromode‐oxyuridine (BrdU), a thymidine analogue, to the culture medium during mid‐to‐late S phase and subsequently staining the chromosomes with 0.1 mg/ml acridine orange. BrdU was added 3.0–10.0 hr postinsemination (hr pi). R bands were obtained when fertilized eggs were transferred into medium containing BrdU between 5.0 and 6.5 hr pi. This indicates that the human (male) pronuclear chromosomes were in mid‐to‐late S phase at these times.

AB - The visualization of human male pronuclear chromosomes is possible by utilizing a technique in which human sperm fertilize zona‐free Golden hamster (Mesocricetus auratus) ova in vitro. R banding of these chromosomes can be achieved by adding 20 μg/ml 5‐bromode‐oxyuridine (BrdU), a thymidine analogue, to the culture medium during mid‐to‐late S phase and subsequently staining the chromosomes with 0.1 mg/ml acridine orange. BrdU was added 3.0–10.0 hr postinsemination (hr pi). R bands were obtained when fertilized eggs were transferred into medium containing BrdU between 5.0 and 6.5 hr pi. This indicates that the human (male) pronuclear chromosomes were in mid‐to‐late S phase at these times.

KW - DNA synthesis

KW - R banding

KW - cross‐species fertilization

KW - human sperm chromosomes

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Timing of human sperm chromosome replication following fertilization of hamster eggs in vitro

Abstract

Keywords

ASJC Scopus subject areas

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