Abstract
The visualization of human male pronuclear chromosomes is possible by utilizing a technique in which human sperm fertilize zona‐free Golden hamster (Mesocricetus auratus) ova in vitro. R banding of these chromosomes can be achieved by adding 20 μg/ml 5‐bromode‐oxyuridine (BrdU), a thymidine analogue, to the culture medium during mid‐to‐late S phase and subsequently staining the chromosomes with 0.1 mg/ml acridine orange. BrdU was added 3.0–10.0 hr postinsemination (hr pi). R bands were obtained when fertilized eggs were transferred into medium containing BrdU between 5.0 and 6.5 hr pi. This indicates that the human (male) pronuclear chromosomes were in mid‐to‐late S phase at these times.
Original language | English (US) |
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Pages (from-to) | 115-119 |
Number of pages | 5 |
Journal | Gamete Research |
Volume | 6 |
Issue number | 2 |
DOIs | |
State | Published - 1982 |
Keywords
- DNA synthesis
- R banding
- cross‐species fertilization
- human sperm chromosomes
ASJC Scopus subject areas
- Genetics
- Developmental Biology