TY - JOUR
T1 - Time-lapse and ultrastructure studies on the reversal of mitotic arrest induced by vinblastine sulfate in earle's l cells
AU - Krishan, Awtar
N1 - Funding Information:
2 Supported in part by Public Health Service research grants CA 6516 from the National Cancer Institute, and FR 05526 from the Division of Research Facilities and Resources, and by the legacy of Loula D. Lasker and the Albert and Mary Lasker Foundation, New York.
PY - 1968/8
Y1 - 1968/8
N2 - L cells exposed to vinblastine sulfate (Velban, Lilly) for 4–24 hours did not show any spindle microtubules in association with centrioles or centromeres. More than one pair of centrioles were seen in many of these cells. Velban-induced mitotic inhibition could be readily removed when cells were washed and reincubated in fresh medium. A large number of these metaphase-blocked and multimicronucleated cells would divide within the first 4 hours after removal of the inhibition. Multipolar chromosome formations, multipolar divisions, and aberrant cytokinesis were seen in many cells released from the mitotic arrest. Aggregates of fine, amorphous, or fibrillar material and large numbers of spindle microtubules were associated with centromeres, chromosomes, and centrioles of cells recovering from the mitotic arrest.
AB - L cells exposed to vinblastine sulfate (Velban, Lilly) for 4–24 hours did not show any spindle microtubules in association with centrioles or centromeres. More than one pair of centrioles were seen in many of these cells. Velban-induced mitotic inhibition could be readily removed when cells were washed and reincubated in fresh medium. A large number of these metaphase-blocked and multimicronucleated cells would divide within the first 4 hours after removal of the inhibition. Multipolar chromosome formations, multipolar divisions, and aberrant cytokinesis were seen in many cells released from the mitotic arrest. Aggregates of fine, amorphous, or fibrillar material and large numbers of spindle microtubules were associated with centromeres, chromosomes, and centrioles of cells recovering from the mitotic arrest.
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U2 - 10.1093/jnci/41.2.581
DO - 10.1093/jnci/41.2.581
M3 - Article
C2 - 5671289
AN - SCOPUS:0014321770
VL - 41
SP - 581
EP - 595
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
SN - 0027-8874
IS - 2
ER -