Time-dependent effects of melatonin on immune measurements in Syrian hamsters

Thomas Champney, Gregg C. Allen, Michael Zannelli, Lee Anne Beausang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Adult, male Syrian hamsters received daily subcutaneous melatonin (25 μg) injections or vehicle injections a 08:00 or 17:00 hr for 11 weeks. Body weights were measured weekly throughout the experiment and testes weights, spleen weights, and serum was collected at the end of the experiment. The spleens were sectioned and immunocytochemically analyzed for immunoglobulin G and serum levels of interferon-gamma, interleukin-2 and interleukin-4 were determined in heterologous mouse assays. Melatonin injections at 17:00 hr, but not at 08:00 hr, increased body weights, decreased testes weights and serum testosterone levels, and had no effect on immunoglobulin G content in the spleen. Likewise, melatonin injections at 17:00 hr, but not at 08:00 hr, increased serum interferon-gamma levels, had no effect on interleukin-2 levels, and appeared to increase interleukin-4 levels. Since melatonin injections at 08:00 hr were ineffective in altering immune measurements and correlations between reproductive measures and immune measures were high, the most parsimonious explanation for these results is that melatonin injections at 17:00 hr depressed reproductive hormone levels and these depressed levels altered immune measures.

Original languageEnglish
Pages (from-to)142-146
Number of pages5
JournalJournal of Pineal Research
Volume25
Issue number3
DOIs
StatePublished - Sep 30 1998
Externally publishedYes

Fingerprint

Mesocricetus
Melatonin
Injections
Spleen
Serum
Weights and Measures
Interleukin-4
Interferon-gamma
Interleukin-2
Testis
Immunoglobulin G
Body Weight
Testosterone
Hormones

Keywords

  • Hamster
  • Immune
  • Interferon
  • Interleukin
  • Melatonin
  • Reproduction

ASJC Scopus subject areas

  • Endocrinology

Cite this

Time-dependent effects of melatonin on immune measurements in Syrian hamsters. / Champney, Thomas; Allen, Gregg C.; Zannelli, Michael; Beausang, Lee Anne.

In: Journal of Pineal Research, Vol. 25, No. 3, 30.09.1998, p. 142-146.

Research output: Contribution to journalArticle

Champney, Thomas ; Allen, Gregg C. ; Zannelli, Michael ; Beausang, Lee Anne. / Time-dependent effects of melatonin on immune measurements in Syrian hamsters. In: Journal of Pineal Research. 1998 ; Vol. 25, No. 3. pp. 142-146.
@article{cc046e4ba3904ff299ce6840e3b4eeb0,
title = "Time-dependent effects of melatonin on immune measurements in Syrian hamsters",
abstract = "Adult, male Syrian hamsters received daily subcutaneous melatonin (25 μg) injections or vehicle injections a 08:00 or 17:00 hr for 11 weeks. Body weights were measured weekly throughout the experiment and testes weights, spleen weights, and serum was collected at the end of the experiment. The spleens were sectioned and immunocytochemically analyzed for immunoglobulin G and serum levels of interferon-gamma, interleukin-2 and interleukin-4 were determined in heterologous mouse assays. Melatonin injections at 17:00 hr, but not at 08:00 hr, increased body weights, decreased testes weights and serum testosterone levels, and had no effect on immunoglobulin G content in the spleen. Likewise, melatonin injections at 17:00 hr, but not at 08:00 hr, increased serum interferon-gamma levels, had no effect on interleukin-2 levels, and appeared to increase interleukin-4 levels. Since melatonin injections at 08:00 hr were ineffective in altering immune measurements and correlations between reproductive measures and immune measures were high, the most parsimonious explanation for these results is that melatonin injections at 17:00 hr depressed reproductive hormone levels and these depressed levels altered immune measures.",
keywords = "Hamster, Immune, Interferon, Interleukin, Melatonin, Reproduction",
author = "Thomas Champney and Allen, {Gregg C.} and Michael Zannelli and Beausang, {Lee Anne}",
year = "1998",
month = "9",
day = "30",
doi = "10.1111/j.1600-079X.1998.tb00552.x",
language = "English",
volume = "25",
pages = "142--146",
journal = "Journal of Pineal Research",
issn = "0742-3098",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Time-dependent effects of melatonin on immune measurements in Syrian hamsters

AU - Champney, Thomas

AU - Allen, Gregg C.

AU - Zannelli, Michael

AU - Beausang, Lee Anne

PY - 1998/9/30

Y1 - 1998/9/30

N2 - Adult, male Syrian hamsters received daily subcutaneous melatonin (25 μg) injections or vehicle injections a 08:00 or 17:00 hr for 11 weeks. Body weights were measured weekly throughout the experiment and testes weights, spleen weights, and serum was collected at the end of the experiment. The spleens were sectioned and immunocytochemically analyzed for immunoglobulin G and serum levels of interferon-gamma, interleukin-2 and interleukin-4 were determined in heterologous mouse assays. Melatonin injections at 17:00 hr, but not at 08:00 hr, increased body weights, decreased testes weights and serum testosterone levels, and had no effect on immunoglobulin G content in the spleen. Likewise, melatonin injections at 17:00 hr, but not at 08:00 hr, increased serum interferon-gamma levels, had no effect on interleukin-2 levels, and appeared to increase interleukin-4 levels. Since melatonin injections at 08:00 hr were ineffective in altering immune measurements and correlations between reproductive measures and immune measures were high, the most parsimonious explanation for these results is that melatonin injections at 17:00 hr depressed reproductive hormone levels and these depressed levels altered immune measures.

AB - Adult, male Syrian hamsters received daily subcutaneous melatonin (25 μg) injections or vehicle injections a 08:00 or 17:00 hr for 11 weeks. Body weights were measured weekly throughout the experiment and testes weights, spleen weights, and serum was collected at the end of the experiment. The spleens were sectioned and immunocytochemically analyzed for immunoglobulin G and serum levels of interferon-gamma, interleukin-2 and interleukin-4 were determined in heterologous mouse assays. Melatonin injections at 17:00 hr, but not at 08:00 hr, increased body weights, decreased testes weights and serum testosterone levels, and had no effect on immunoglobulin G content in the spleen. Likewise, melatonin injections at 17:00 hr, but not at 08:00 hr, increased serum interferon-gamma levels, had no effect on interleukin-2 levels, and appeared to increase interleukin-4 levels. Since melatonin injections at 08:00 hr were ineffective in altering immune measurements and correlations between reproductive measures and immune measures were high, the most parsimonious explanation for these results is that melatonin injections at 17:00 hr depressed reproductive hormone levels and these depressed levels altered immune measures.

KW - Hamster

KW - Immune

KW - Interferon

KW - Interleukin

KW - Melatonin

KW - Reproduction

UR - http://www.scopus.com/inward/record.url?scp=0031680530&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031680530&partnerID=8YFLogxK

U2 - 10.1111/j.1600-079X.1998.tb00552.x

DO - 10.1111/j.1600-079X.1998.tb00552.x

M3 - Article

VL - 25

SP - 142

EP - 146

JO - Journal of Pineal Research

JF - Journal of Pineal Research

SN - 0742-3098

IS - 3

ER -