Time-dependent alterations of myocardial microsomal yield and calcium accumulation in experimentally-induced right ventricular hypertrophy and failure

Marshal Shlafer, Henry Gelband, Ruey J. Sung, Roger F. Palmer, Arthur L. Bassett

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

A preliminary study was designed to determine whether time-dependent alterations of cardiac microsomal yield and in vitro Ca2+-accumulating activity occur during the development of pressure-overload-induced right vetricular hypertrophy (RVH). RVH was produced in cats by pulmonary artery banding, and they were killed 3 days ("early" RVH) or 30 or more days ("long-term" RVH) later. Microsomes were isolated by differential centrifugation of right ventricular free wall (RVFW) and left ventricular wall plus septum (LVS) homogenates. RVFW microsomal yield was significantly greater in 3-day RVH than in normal, sham-operated or long-term RVH cats. Under some experimental conditions, Ca2+ accumulation by RVFW microsomes from RVH cats was significantly different from that seen in other groups; the duration of pressure overload did not alter maximum Ca2+ accumulation significantly. RVFW microsomal Ca2+ accumulation was markedly depressed in cats with RVH complicated by right ventricular failure. LVS microsomal yield and Ca2+ accumulation were not altered significantly in any group. Our microsomal data suggests that in this model early RVH involves rapid compensatory synthesis of RVFW sarcoplasmic reticulum, with some increase in the ability of these membranes to accumulate Ca2+. Inability to maintain compensated RVH, resulting in failure, appears to involve compromised Ca2+ accumulation, but no apparent impairment of microsomal protein synthesis.

Original languageEnglish (US)
Pages (from-to)395-407
Number of pages13
JournalJournal of Molecular and Cellular Cardiology
Volume10
Issue number4
DOIs
StatePublished - Apr 1978

Keywords

  • Ca accumulation
  • Microsomes
  • Protein synthesis
  • Sarcoplasmic reticulum
  • Ventricular failure
  • Ventricular hypertrophy

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Time-dependent alterations of myocardial microsomal yield and calcium accumulation in experimentally-induced right ventricular hypertrophy and failure'. Together they form a unique fingerprint.

Cite this