Time- and Oil-Dependent Transcriptomic and Physiological Responses to Deepwater Horizon Oil in Mahi-Mahi (Coryphaena hippurus) Embryos and Larvae

Elvis Genbo Xu, Edward M. Mager, Martin Grosell, Christina Pasparakis, Lela S. Schlenker, John Stieglitz, Daniel D Benetti, E. Starr Hazard, Sean M. Courtney, Graciel Diamante, Juliane Freitas, Gary Hardiman, Daniel Schlenk

Research output: Contribution to journalArticle

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Abstract

The Deepwater Horizon (DWH) oil spill contaminated the spawning habitats for numerous commercially and ecologically important fishes. Exposure to the water accommodated fraction (WAF) of oil from the spill has been shown to cause cardiac toxicity during early developmental stages across fishes. To better understand the molecular events and explore new pathways responsible for toxicity, RNA sequencing was performed in conjunction with physiological and morphological assessments to analyze the time-course (24, 48, and 96 h post fertilization (hpf)) of transcriptional and developmental responses in embryos/larvae of mahi-mahi exposed to WAF of weathered (slick) and source DWH oils. Slick oil exposure induced more pronounced changes in gene expression over time than source oil exposure. Predominant transcriptomic responses included alteration of EIF2 signaling, steroid biosynthesis, ribosome biogenesis and activation of the cytochrome P450 pathway. At 96 hpf, slick oil exposure resulted in significant perturbations in eye development and peripheral nervous system, suggesting novel targets in addition to the heart may be involved in the developmental toxicity of DHW oil. Comparisons of changes of cardiac genes with phenotypic responses were consistent with reduced heart rate and increased pericardial edema in larvae exposed to slick oil but not source oil.

Original languageEnglish (US)
Pages (from-to)7842-7851
Number of pages10
JournalEnvironmental Science and Technology
Volume50
Issue number14
DOIs
StatePublished - Jul 19 2016

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physiological response
embryo
Oils
larva
oil
toxicity
Toxicity
Fish
nervous system
steroid
fish
developmental stage
oil spill
Eukaryotic Initiation Factor-2
gene expression
cytochrome
RNA
spawning
perturbation
Water

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Time- and Oil-Dependent Transcriptomic and Physiological Responses to Deepwater Horizon Oil in Mahi-Mahi (Coryphaena hippurus) Embryos and Larvae. / Xu, Elvis Genbo; Mager, Edward M.; Grosell, Martin; Pasparakis, Christina; Schlenker, Lela S.; Stieglitz, John; Benetti, Daniel D; Hazard, E. Starr; Courtney, Sean M.; Diamante, Graciel; Freitas, Juliane; Hardiman, Gary; Schlenk, Daniel.

In: Environmental Science and Technology, Vol. 50, No. 14, 19.07.2016, p. 7842-7851.

Research output: Contribution to journalArticle

Xu, Elvis Genbo ; Mager, Edward M. ; Grosell, Martin ; Pasparakis, Christina ; Schlenker, Lela S. ; Stieglitz, John ; Benetti, Daniel D ; Hazard, E. Starr ; Courtney, Sean M. ; Diamante, Graciel ; Freitas, Juliane ; Hardiman, Gary ; Schlenk, Daniel. / Time- and Oil-Dependent Transcriptomic and Physiological Responses to Deepwater Horizon Oil in Mahi-Mahi (Coryphaena hippurus) Embryos and Larvae. In: Environmental Science and Technology. 2016 ; Vol. 50, No. 14. pp. 7842-7851.
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