TY - JOUR
T1 - Thyroid transcription factor 1
T2 - A marker for lung adenocarcinoma in body cavity fluids
AU - Gomez-Fernandez, Carmen
AU - Jorda, Merce
AU - Delgado, Patricia I.
AU - Ganjei-Azar, Parvin
PY - 2002/10/25
Y1 - 2002/10/25
N2 - BACKGROUND. Adenocarcinomas are the most common epithelial malignancies in body cavity fluids. Subclassification of adenocarcinomas according to primary site can be a challenging task. Thyroid transcription factor 1 (TTF-1) is a nuclear transcription factor that is expressed in normal lung, in thyroid, and in their neoplasms. Because thyroid carcinomas rarely metastasize to the serosal surfaces, the authors used TTF-1 as a marker to distinguish adenocarcinomas of the lung from carcinomas of other organs. METHODS. The authors studied 113 body cavity fluids (92 pleural fluid samples and 21 ascitic samples) from 113 patients with a diagnosis of adenocarcinoma on the basis of routine cytology. The primary sites of origin were confirmed clinically or histologically for all patients. There were 39 adenocarcinomas of the lung, 24 adenocarcinomas of the breast, 34 adenocarcinomas of the genitourinary tract, and 16 adenocarcinomas of the gastrointestinal tract. Archival Papanicolaoustained, cytocentrifuged slides were used without destaining for immunocyto-chemistry with the monoclonal TTF-1 antibody using a commercial available method. RESULTS. TTF-1 was expressed in 21 of 39 lung adenocarcinomas (54%). Intense nuclear staining was present in tumor cells that occurred in groups or in isolated form. In contrast, none of the other types of adenocarcinomas expressed TTF-1. CONCLUSIONS. TTF-1 is a highly specific marker for adenocarcinomas of the lung in body cavity fluids. Immunocytochemistry using this antibody can be performed easily on archival Papanicolaou-stained, cytocentrifuged slides of fluid specimens.
AB - BACKGROUND. Adenocarcinomas are the most common epithelial malignancies in body cavity fluids. Subclassification of adenocarcinomas according to primary site can be a challenging task. Thyroid transcription factor 1 (TTF-1) is a nuclear transcription factor that is expressed in normal lung, in thyroid, and in their neoplasms. Because thyroid carcinomas rarely metastasize to the serosal surfaces, the authors used TTF-1 as a marker to distinguish adenocarcinomas of the lung from carcinomas of other organs. METHODS. The authors studied 113 body cavity fluids (92 pleural fluid samples and 21 ascitic samples) from 113 patients with a diagnosis of adenocarcinoma on the basis of routine cytology. The primary sites of origin were confirmed clinically or histologically for all patients. There were 39 adenocarcinomas of the lung, 24 adenocarcinomas of the breast, 34 adenocarcinomas of the genitourinary tract, and 16 adenocarcinomas of the gastrointestinal tract. Archival Papanicolaoustained, cytocentrifuged slides were used without destaining for immunocyto-chemistry with the monoclonal TTF-1 antibody using a commercial available method. RESULTS. TTF-1 was expressed in 21 of 39 lung adenocarcinomas (54%). Intense nuclear staining was present in tumor cells that occurred in groups or in isolated form. In contrast, none of the other types of adenocarcinomas expressed TTF-1. CONCLUSIONS. TTF-1 is a highly specific marker for adenocarcinomas of the lung in body cavity fluids. Immunocytochemistry using this antibody can be performed easily on archival Papanicolaou-stained, cytocentrifuged slides of fluid specimens.
KW - Body cavity fluids
KW - Effusion cytology
KW - Immunocytochemistry
KW - Lung carcinoma
KW - Thyroid transcription factor 1
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U2 - 10.1002/cncr.10743
DO - 10.1002/cncr.10743
M3 - Article
C2 - 12378596
AN - SCOPUS:0037174795
VL - 96
SP - 289
EP - 293
JO - Cancer
JF - Cancer
SN - 0008-543X
IS - 5
ER -