Thrombospondin-related adhesive protein (TRAP) of Plasmodium falciparum: Expression during sporozoite ontogeny and binding to human hepatocytes

K. J H Robson, U. Frevert, I. Reckmann, G. Cowan, John C Beier, I. G. Scragg, K. Takehara, D. H L Bishop, G. Pradel, R. Sinden, S. Saccheo, H. M. Muller, A. Crisanti

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Plasmodium sporozoites collected from oocysts, haemocoel and salivary glands of the mosquito show profound differences in their biological properties such as motility, ability to induce protective immune response and infectivity for vertebrate host cells. Sporozoites from salivary glands are much more infectious than those from oocysts and haemocoel. Differential expression of proteins, such as the circumsporozoite (CS) protein and the thrombospondin-related adhesive protein (TRAP), implicated in sporozoite recognition and entry into hepatocytes may account for the development of infectivity during ontogeny. We have carried out a series of experiments to: (i) analyse the expression and localization of TRAP in P.falciparum sporozoites during development in the mosquito; and (ii) elucidate the biochemical and adhesive properties of recombinant TRAP. Our data indicate that TRAP is not expressed in oocysts, whereas variable amounts of CS protein are found in this parasite developmental stage. Hemocoel sporozoites display the distinct phenotypes TRAP- CS protein+ and TRAP+ CS protein+ at a frequency of 98.5 and 1.5% respectively. Salivary gland sporozoites are all TRAP+ CS protein+. We also provide experimental evidence showing that recombinant TRAP binds to the basolateral cell membrane of hepatocytes in the Disse's space and that sulfated glycoconjugates function as TRAP ligands on human hepatocytes.

Original languageEnglish
Pages (from-to)3883-3894
Number of pages12
JournalEMBO Journal
Volume14
Issue number16
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Thrombospondins
Sporozoites
Plasmodium falciparum
Adhesives
Hepatocytes
Proteins
Oocysts
Salivary Glands
Culicidae
Glycoconjugates
Plasmodium
Cell membranes

Keywords

  • Hepatocyte binding
  • Plasmodium falciparum
  • Sporozoite ontogeny
  • TRAP expression

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Robson, K. J. H., Frevert, U., Reckmann, I., Cowan, G., Beier, J. C., Scragg, I. G., ... Crisanti, A. (1995). Thrombospondin-related adhesive protein (TRAP) of Plasmodium falciparum: Expression during sporozoite ontogeny and binding to human hepatocytes. EMBO Journal, 14(16), 3883-3894.

Thrombospondin-related adhesive protein (TRAP) of Plasmodium falciparum : Expression during sporozoite ontogeny and binding to human hepatocytes. / Robson, K. J H; Frevert, U.; Reckmann, I.; Cowan, G.; Beier, John C; Scragg, I. G.; Takehara, K.; Bishop, D. H L; Pradel, G.; Sinden, R.; Saccheo, S.; Muller, H. M.; Crisanti, A.

In: EMBO Journal, Vol. 14, No. 16, 01.01.1995, p. 3883-3894.

Research output: Contribution to journalArticle

Robson, KJH, Frevert, U, Reckmann, I, Cowan, G, Beier, JC, Scragg, IG, Takehara, K, Bishop, DHL, Pradel, G, Sinden, R, Saccheo, S, Muller, HM & Crisanti, A 1995, 'Thrombospondin-related adhesive protein (TRAP) of Plasmodium falciparum: Expression during sporozoite ontogeny and binding to human hepatocytes', EMBO Journal, vol. 14, no. 16, pp. 3883-3894.
Robson, K. J H ; Frevert, U. ; Reckmann, I. ; Cowan, G. ; Beier, John C ; Scragg, I. G. ; Takehara, K. ; Bishop, D. H L ; Pradel, G. ; Sinden, R. ; Saccheo, S. ; Muller, H. M. ; Crisanti, A. / Thrombospondin-related adhesive protein (TRAP) of Plasmodium falciparum : Expression during sporozoite ontogeny and binding to human hepatocytes. In: EMBO Journal. 1995 ; Vol. 14, No. 16. pp. 3883-3894.
@article{e0ff422d9a244992a81c11715cd87354,
title = "Thrombospondin-related adhesive protein (TRAP) of Plasmodium falciparum: Expression during sporozoite ontogeny and binding to human hepatocytes",
abstract = "Plasmodium sporozoites collected from oocysts, haemocoel and salivary glands of the mosquito show profound differences in their biological properties such as motility, ability to induce protective immune response and infectivity for vertebrate host cells. Sporozoites from salivary glands are much more infectious than those from oocysts and haemocoel. Differential expression of proteins, such as the circumsporozoite (CS) protein and the thrombospondin-related adhesive protein (TRAP), implicated in sporozoite recognition and entry into hepatocytes may account for the development of infectivity during ontogeny. We have carried out a series of experiments to: (i) analyse the expression and localization of TRAP in P.falciparum sporozoites during development in the mosquito; and (ii) elucidate the biochemical and adhesive properties of recombinant TRAP. Our data indicate that TRAP is not expressed in oocysts, whereas variable amounts of CS protein are found in this parasite developmental stage. Hemocoel sporozoites display the distinct phenotypes TRAP- CS protein+ and TRAP+ CS protein+ at a frequency of 98.5 and 1.5{\%} respectively. Salivary gland sporozoites are all TRAP+ CS protein+. We also provide experimental evidence showing that recombinant TRAP binds to the basolateral cell membrane of hepatocytes in the Disse's space and that sulfated glycoconjugates function as TRAP ligands on human hepatocytes.",
keywords = "Hepatocyte binding, Plasmodium falciparum, Sporozoite ontogeny, TRAP expression",
author = "Robson, {K. J H} and U. Frevert and I. Reckmann and G. Cowan and Beier, {John C} and Scragg, {I. G.} and K. Takehara and Bishop, {D. H L} and G. Pradel and R. Sinden and S. Saccheo and Muller, {H. M.} and A. Crisanti",
year = "1995",
month = "1",
day = "1",
language = "English",
volume = "14",
pages = "3883--3894",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "16",

}

TY - JOUR

T1 - Thrombospondin-related adhesive protein (TRAP) of Plasmodium falciparum

T2 - Expression during sporozoite ontogeny and binding to human hepatocytes

AU - Robson, K. J H

AU - Frevert, U.

AU - Reckmann, I.

AU - Cowan, G.

AU - Beier, John C

AU - Scragg, I. G.

AU - Takehara, K.

AU - Bishop, D. H L

AU - Pradel, G.

AU - Sinden, R.

AU - Saccheo, S.

AU - Muller, H. M.

AU - Crisanti, A.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Plasmodium sporozoites collected from oocysts, haemocoel and salivary glands of the mosquito show profound differences in their biological properties such as motility, ability to induce protective immune response and infectivity for vertebrate host cells. Sporozoites from salivary glands are much more infectious than those from oocysts and haemocoel. Differential expression of proteins, such as the circumsporozoite (CS) protein and the thrombospondin-related adhesive protein (TRAP), implicated in sporozoite recognition and entry into hepatocytes may account for the development of infectivity during ontogeny. We have carried out a series of experiments to: (i) analyse the expression and localization of TRAP in P.falciparum sporozoites during development in the mosquito; and (ii) elucidate the biochemical and adhesive properties of recombinant TRAP. Our data indicate that TRAP is not expressed in oocysts, whereas variable amounts of CS protein are found in this parasite developmental stage. Hemocoel sporozoites display the distinct phenotypes TRAP- CS protein+ and TRAP+ CS protein+ at a frequency of 98.5 and 1.5% respectively. Salivary gland sporozoites are all TRAP+ CS protein+. We also provide experimental evidence showing that recombinant TRAP binds to the basolateral cell membrane of hepatocytes in the Disse's space and that sulfated glycoconjugates function as TRAP ligands on human hepatocytes.

AB - Plasmodium sporozoites collected from oocysts, haemocoel and salivary glands of the mosquito show profound differences in their biological properties such as motility, ability to induce protective immune response and infectivity for vertebrate host cells. Sporozoites from salivary glands are much more infectious than those from oocysts and haemocoel. Differential expression of proteins, such as the circumsporozoite (CS) protein and the thrombospondin-related adhesive protein (TRAP), implicated in sporozoite recognition and entry into hepatocytes may account for the development of infectivity during ontogeny. We have carried out a series of experiments to: (i) analyse the expression and localization of TRAP in P.falciparum sporozoites during development in the mosquito; and (ii) elucidate the biochemical and adhesive properties of recombinant TRAP. Our data indicate that TRAP is not expressed in oocysts, whereas variable amounts of CS protein are found in this parasite developmental stage. Hemocoel sporozoites display the distinct phenotypes TRAP- CS protein+ and TRAP+ CS protein+ at a frequency of 98.5 and 1.5% respectively. Salivary gland sporozoites are all TRAP+ CS protein+. We also provide experimental evidence showing that recombinant TRAP binds to the basolateral cell membrane of hepatocytes in the Disse's space and that sulfated glycoconjugates function as TRAP ligands on human hepatocytes.

KW - Hepatocyte binding

KW - Plasmodium falciparum

KW - Sporozoite ontogeny

KW - TRAP expression

UR - http://www.scopus.com/inward/record.url?scp=0029091846&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029091846&partnerID=8YFLogxK

M3 - Article

C2 - 7664729

AN - SCOPUS:0029091846

VL - 14

SP - 3883

EP - 3894

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 16

ER -