Abstract
Plasmodium sporozoites collected from oocysts, haemocoel and salivary glands of the mosquito show profound differences in their biological properties such as motility, ability to induce protective immune response and infectivity for vertebrate host cells. Sporozoites from salivary glands are much more infectious than those from oocysts and haemocoel. Differential expression of proteins, such as the circumsporozoite (CS) protein and the thrombospondin-related adhesive protein (TRAP), implicated in sporozoite recognition and entry into hepatocytes may account for the development of infectivity during ontogeny. We have carried out a series of experiments to: (i) analyse the expression and localization of TRAP in P.falciparum sporozoites during development in the mosquito; and (ii) elucidate the biochemical and adhesive properties of recombinant TRAP. Our data indicate that TRAP is not expressed in oocysts, whereas variable amounts of CS protein are found in this parasite developmental stage. Hemocoel sporozoites display the distinct phenotypes TRAP- CS protein+ and TRAP+ CS protein+ at a frequency of 98.5 and 1.5% respectively. Salivary gland sporozoites are all TRAP+ CS protein+. We also provide experimental evidence showing that recombinant TRAP binds to the basolateral cell membrane of hepatocytes in the Disse's space and that sulfated glycoconjugates function as TRAP ligands on human hepatocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 3883-3894 |
| Number of pages | 12 |
| Journal | EMBO Journal |
| Volume | 14 |
| Issue number | 16 |
| State | Published - Jan 1 1995 |
| Externally published | Yes |
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Keywords
- Hepatocyte binding
- Plasmodium falciparum
- Sporozoite ontogeny
- TRAP expression
ASJC Scopus subject areas
- Cell Biology
- Genetics
Cite this
Thrombospondin-related adhesive protein (TRAP) of Plasmodium falciparum : Expression during sporozoite ontogeny and binding to human hepatocytes. / Robson, K. J H; Frevert, U.; Reckmann, I.; Cowan, G.; Beier, John C; Scragg, I. G.; Takehara, K.; Bishop, D. H L; Pradel, G.; Sinden, R.; Saccheo, S.; Muller, H. M.; Crisanti, A.
In: EMBO Journal, Vol. 14, No. 16, 01.01.1995, p. 3883-3894.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Thrombospondin-related adhesive protein (TRAP) of Plasmodium falciparum
T2 - Expression during sporozoite ontogeny and binding to human hepatocytes
AU - Robson, K. J H
AU - Frevert, U.
AU - Reckmann, I.
AU - Cowan, G.
AU - Beier, John C
AU - Scragg, I. G.
AU - Takehara, K.
AU - Bishop, D. H L
AU - Pradel, G.
AU - Sinden, R.
AU - Saccheo, S.
AU - Muller, H. M.
AU - Crisanti, A.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - Plasmodium sporozoites collected from oocysts, haemocoel and salivary glands of the mosquito show profound differences in their biological properties such as motility, ability to induce protective immune response and infectivity for vertebrate host cells. Sporozoites from salivary glands are much more infectious than those from oocysts and haemocoel. Differential expression of proteins, such as the circumsporozoite (CS) protein and the thrombospondin-related adhesive protein (TRAP), implicated in sporozoite recognition and entry into hepatocytes may account for the development of infectivity during ontogeny. We have carried out a series of experiments to: (i) analyse the expression and localization of TRAP in P.falciparum sporozoites during development in the mosquito; and (ii) elucidate the biochemical and adhesive properties of recombinant TRAP. Our data indicate that TRAP is not expressed in oocysts, whereas variable amounts of CS protein are found in this parasite developmental stage. Hemocoel sporozoites display the distinct phenotypes TRAP- CS protein+ and TRAP+ CS protein+ at a frequency of 98.5 and 1.5% respectively. Salivary gland sporozoites are all TRAP+ CS protein+. We also provide experimental evidence showing that recombinant TRAP binds to the basolateral cell membrane of hepatocytes in the Disse's space and that sulfated glycoconjugates function as TRAP ligands on human hepatocytes.
AB - Plasmodium sporozoites collected from oocysts, haemocoel and salivary glands of the mosquito show profound differences in their biological properties such as motility, ability to induce protective immune response and infectivity for vertebrate host cells. Sporozoites from salivary glands are much more infectious than those from oocysts and haemocoel. Differential expression of proteins, such as the circumsporozoite (CS) protein and the thrombospondin-related adhesive protein (TRAP), implicated in sporozoite recognition and entry into hepatocytes may account for the development of infectivity during ontogeny. We have carried out a series of experiments to: (i) analyse the expression and localization of TRAP in P.falciparum sporozoites during development in the mosquito; and (ii) elucidate the biochemical and adhesive properties of recombinant TRAP. Our data indicate that TRAP is not expressed in oocysts, whereas variable amounts of CS protein are found in this parasite developmental stage. Hemocoel sporozoites display the distinct phenotypes TRAP- CS protein+ and TRAP+ CS protein+ at a frequency of 98.5 and 1.5% respectively. Salivary gland sporozoites are all TRAP+ CS protein+. We also provide experimental evidence showing that recombinant TRAP binds to the basolateral cell membrane of hepatocytes in the Disse's space and that sulfated glycoconjugates function as TRAP ligands on human hepatocytes.
KW - Hepatocyte binding
KW - Plasmodium falciparum
KW - Sporozoite ontogeny
KW - TRAP expression
UR - http://www.scopus.com/inward/record.url?scp=0029091846&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029091846&partnerID=8YFLogxK
M3 - Article
VL - 14
SP - 3883
EP - 3894
JO - EMBO Journal
JF - EMBO Journal
SN - 0261-4189
IS - 16
ER -