Three distinct messenger RNA distribution patterns in human jejunal enterocytes

Wei Li, S. D. Krasinski, M. Verhave, R. K. Montgomery, R. J. Grand

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background and Aims: The importance of messenger RNA (mRNA) localization in human enterocytes is poorly understood. Previous studies from our laboratory have indicated that mRNAs are asymmetrically distributed in human intestinal epithelial cells, but in general colocalized with their encoded proteins. The aim of this study was to characterize, in human enterocytes, mRNA localization patterns of three genes with distinctly different functions. Methods: mRNA distribution was determined by in situ hybridization with digoxigenin-labeled RNA probes in tissue sections of human jejunum. Results: The mRNA for villin, a well-characterized microvillus cytoskeletal protein, was sorted to the basal region of the enterocyte. The mRNA for human sodium glucose cotransporter 1 was localized to the apical region, and the mRNA for human liver fatty acid-binding protein was distributed diffusely in the cytoplasm. Conclusions: The three distinct mRNA distribution patterns suggest that active mRNA sorting mechanisms exist in human enterocytes. This study also reveals for the first time that dichotomies may occur between the distribution patterns of sorted mRNAs and their encoded proteins.

Original languageEnglish (US)
Pages (from-to)86-92
Number of pages7
JournalGastroenterology
Volume115
Issue number1
DOIs
StatePublished - 1998
Externally publishedYes

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Enterocytes
Messenger RNA
Sodium-Glucose Transporter 1
RNA Transport
RNA Probes
Digoxigenin
Fatty Acid-Binding Proteins
Cytoskeletal Proteins
Jejunum
Microvilli
In Situ Hybridization
Cytoplasm
Proteins
Epithelial Cells

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Three distinct messenger RNA distribution patterns in human jejunal enterocytes. / Li, Wei; Krasinski, S. D.; Verhave, M.; Montgomery, R. K.; Grand, R. J.

In: Gastroenterology, Vol. 115, No. 1, 1998, p. 86-92.

Research output: Contribution to journalArticle

Li, Wei ; Krasinski, S. D. ; Verhave, M. ; Montgomery, R. K. ; Grand, R. J. / Three distinct messenger RNA distribution patterns in human jejunal enterocytes. In: Gastroenterology. 1998 ; Vol. 115, No. 1. pp. 86-92.
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