Three-dimensional structure of a macromolecular assembly that regulates type III secretion in Yersinia pestis

Florian D. Schubot, Michael W. Jackson, Kerri J. Penrose, Scott Cherry, Joseph E. Tropea, Gregory V Plano, David S. Waugh

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Yersinia pestis, the causative agent of plague, utilizes a type III secretion system (T3SS) to inject effector proteins directly into the cytosol of mammalian cells where they interfere with signal transduction pathways that regulate actin cytoskeleton dynamics and inflammation, thereby enabling the bacterium to avoid engulfment and destruction by macrophages. Type III secretion normally does not occur in the absence of close contact with eukaryotic cells. Negative regulation is mediated in part by a multiprotein complex that has been proposed to act as a physical impediment to type III secretion by blocking the entrance to the secretion apparatus prior to contact with mammalian cells. This complex is composed of YopN, its heterodimeric secretion chaperone SycN-YscB, and TyeA. Here, we report two crystal structures of YopN in complex with its heterodimeric secretion chaperone SycN-YscB and the co-regulatory protein TyeA, respectively. By merging these two overlapping structures, it was possible to construct a credible theoretical model of the YopN-SycN-YscB-TyeA complex. The modeled assembly features the secretion signaling elements of YopN at one end of an elongated structure and the secretion regulating TyeA binding site at the other. A patch of highly conserved residues on the surface of the C-terminal α-helix of TyeA may mediate its interaction with structural components of the secretion apparatus. Conserved arginine residues that reside inside a prominent cavity at the dimer interface of SycN-YscB were mutated in order to investigate whether they play a role in targeting the YopN-chaperone complex to the type III secretion apparatus. One of the mutants exhibited a phenotype that is consistent with this hypothesis.

Original languageEnglish
Pages (from-to)1147-1161
Number of pages15
JournalJournal of Molecular Biology
Volume346
Issue number4
DOIs
StatePublished - Mar 4 2005

Fingerprint

Yersinia pestis
Multiprotein Complexes
Plague
Eukaryotic Cells
Actin Cytoskeleton
Cytosol
Arginine
Signal Transduction
Proteins
Theoretical Models
Macrophages
Binding Sites
Inflammation
Bacteria
Phenotype
Type III Secretion Systems

Keywords

  • SycN
  • Tye A
  • Type III secretion
  • YopN
  • YscB

ASJC Scopus subject areas

  • Virology

Cite this

Three-dimensional structure of a macromolecular assembly that regulates type III secretion in Yersinia pestis. / Schubot, Florian D.; Jackson, Michael W.; Penrose, Kerri J.; Cherry, Scott; Tropea, Joseph E.; Plano, Gregory V; Waugh, David S.

In: Journal of Molecular Biology, Vol. 346, No. 4, 04.03.2005, p. 1147-1161.

Research output: Contribution to journalArticle

Schubot, Florian D. ; Jackson, Michael W. ; Penrose, Kerri J. ; Cherry, Scott ; Tropea, Joseph E. ; Plano, Gregory V ; Waugh, David S. / Three-dimensional structure of a macromolecular assembly that regulates type III secretion in Yersinia pestis. In: Journal of Molecular Biology. 2005 ; Vol. 346, No. 4. pp. 1147-1161.
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