Three-dimensional migration of neurites is mediated by adhesion site density and affinity

Jason C. Schense, Jeffrey A. Hubbell

Research output: Contribution to journalArticlepeer-review

143 Scopus citations


Three-dimensional neurite outgrowth rates within fibrin matrices that contained variable amounts of RGD peptides were shown to depend on adhesion site density and affinity. Bi-domain peptides with a factor XIIIa substrate in one domain and a RGD sequence in the other domain were covalently incorporated into fibrin gels during coagulation through the action of the transglutaminase factor XIIIa, and the RGD-dependent effect on neurite outgrowth was quantified, employing chick dorsal root ganglia cultured two- and three-dimensionally within the modified fibrin. Two separate bi-domain peptides were synthesized, one with a lower binding affinity linear RGD domain and another with a higher binding affinity cyclic RGD domain. Both peptides were crosslinked into fibrin gels at concentrations up to 8.2 tool of peptide/tool of fibrinogen, and their effect on neurite outgrowth was measured. Both two- and three-dimensional neurite outgrowth demonstrated a bi-phasic dependence on RGD concentration for both the linear and cyclic peptide, with intermediate adhesion site densities yielding maximal neurite extension and higher densities inhibiting outgrowth. The adhesion site density that yielded maximal outgrowth depended strongly on adhesion site affinity in both two and three dimensions, with lower densities of the higher affinity ligand being required (0.8-1.7 tool/tool for the linear peptide versus 0.2 tool/tool for the cyclic peptide yielding maximum neurite outgrowth rates in three-dimensional cultures).

Original languageEnglish (US)
Pages (from-to)6813-6818
Number of pages6
JournalJournal of Biological Chemistry
Issue number10
StatePublished - Mar 10 2000
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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