Three active forms of aspartic proteinase from Mason-Pfizer monkey virus

Aleš Zábranský, Martin Andreánsky, Olga Hrušková-Heidingsfeldová, Vladimír Havlíček, Eric Hunter, Tomáš Ruml, Iva Pichová

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Mason-Pfizer monkey virus (M-PMV) proteinase, released by the autocatalytic cleavage of Gag-Pro and Gag-Pro-Pol polypeptide precursors, catalyzes the processing of viral precursors to yield the structural proteins and enzymes of the virion. In retroviruses, usually only one proteolytically active form of proteinase exists. Here, we describe an unusual feature of M- PMV, the existence of three active forms of a retroviral proteinase with molecular masses of 17, 13, and 12 kDa as determined by mass spectroscopy. These forms arise in vitro by self-processing of a 26-kDa proteinase precursor. We have developed a process for isolation of each truncated product and demonstrate that all three forms display proteolytic activity. Amino acid analyses, as well as the determination of N- and C-terminal sequences, revealed that the N-termini of all three forms are identical, confirming that in vitro autoprocessing of the 17-kDa form occurs at the C- terminus to yield the truncated forms. The 17-kDa form and the newly described 13-kDa form of proteinase were identified in virions collected from the rhesus monkey CMMT cell line chronically infected with M-PMV, confirming that multiple forms exist in vivo.

Original languageEnglish (US)
Pages (from-to)250-256
Number of pages7
JournalVirology
Volume245
Issue number2
DOIs
StatePublished - Jun 5 1998
Externally publishedYes

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Three active forms of aspartic proteinase from Mason-Pfizer monkey virus'. Together they form a unique fingerprint.

  • Cite this

    Zábranský, A., Andreánsky, M., Hrušková-Heidingsfeldová, O., Havlíček, V., Hunter, E., Ruml, T., & Pichová, I. (1998). Three active forms of aspartic proteinase from Mason-Pfizer monkey virus. Virology, 245(2), 250-256. https://doi.org/10.1006/viro.1998.9173