The yeast SIN3 gene product negatively regulates the activity of the human progesterone receptor and positively regulates the activities of GAL4 and the HAP1 activator

Zafar Nawaz, Claudia Baniahmad, Thomas P. Burris, Bert W. O'Malley, David J. Stillman, Ming Jer TsaiTsaiTsai

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The activation of gene transcription in eukaryotic organisms is regulated by sequence-specific DNA-binding proteins as well as by non-DNA-binding proteins. In this report we describe the modulatory functions of a non-DNA-binding protein, SIN3 (also known as SDI1, UME4, RPD1, and GAM2) on the transactivation properties of the human progesterone receptor (hPR), GAL4, and the HAPl activator in yeast. Our data suggest that SIN3 is a dual function protein. It negatively regulates the transcriptional activities of hPR-A and hPR-B by affecting the N-terminal activation domain (AFI). SIN3 positively regulates the transcriptional activities of GAL4 and the HAP1 activator. However, it has no effect on the transcriptional activities of the human glucocorticoid receptor (hGR) and GCN4. The SIN3 protein contains four copies of a paired amphipathic helix (PAH) motif. Deletion analysis of the SIN3 PAH motifs shows that the PAH3 motif is essential for SIN3-mediated regulation of hPR, GAL4, and the HAPI activator. In constrast, the PAH 1, PAH2, and PAH4 motifs are not required for SIN3-mediated regulation of these activators. Additionally, we examined the mechanism(s) by which the SIN3 protein modulate the activities of various activators. We are unable to demonstrate the direct interaction of SIN3 protein with these activators using the yeast two-hybrid system or co-immunoprecipitation. These data suggest that SIN3 regulates the transactivation functions of hPR, GAL4, and the HAP1 activator by an indirect mechanism.

Original languageEnglish
Pages (from-to)724-733
Number of pages10
JournalMGG Molecular & General Genetics
Volume245
Issue number6
DOIs
StatePublished - Nov 1 1994
Externally publishedYes

Fingerprint

Progesterone Receptors
Human Activities
Transcriptional Activation
Yeasts
Genes
Carrier Proteins
Proteins
Two-Hybrid System Techniques
Glucocorticoid Receptors
DNA-Binding Proteins
Immunoprecipitation

Keywords

  • GAL4
  • Gene regulation
  • HAP1
  • SIN3 protein
  • Transcription

ASJC Scopus subject areas

  • Genetics

Cite this

The yeast SIN3 gene product negatively regulates the activity of the human progesterone receptor and positively regulates the activities of GAL4 and the HAP1 activator. / Nawaz, Zafar; Baniahmad, Claudia; Burris, Thomas P.; O'Malley, Bert W.; Stillman, David J.; TsaiTsaiTsai, Ming Jer.

In: MGG Molecular & General Genetics, Vol. 245, No. 6, 01.11.1994, p. 724-733.

Research output: Contribution to journalArticle

Nawaz, Zafar ; Baniahmad, Claudia ; Burris, Thomas P. ; O'Malley, Bert W. ; Stillman, David J. ; TsaiTsaiTsai, Ming Jer. / The yeast SIN3 gene product negatively regulates the activity of the human progesterone receptor and positively regulates the activities of GAL4 and the HAP1 activator. In: MGG Molecular & General Genetics. 1994 ; Vol. 245, No. 6. pp. 724-733.
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AU - Stillman, David J.

AU - TsaiTsaiTsai, Ming Jer

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AB - The activation of gene transcription in eukaryotic organisms is regulated by sequence-specific DNA-binding proteins as well as by non-DNA-binding proteins. In this report we describe the modulatory functions of a non-DNA-binding protein, SIN3 (also known as SDI1, UME4, RPD1, and GAM2) on the transactivation properties of the human progesterone receptor (hPR), GAL4, and the HAPl activator in yeast. Our data suggest that SIN3 is a dual function protein. It negatively regulates the transcriptional activities of hPR-A and hPR-B by affecting the N-terminal activation domain (AFI). SIN3 positively regulates the transcriptional activities of GAL4 and the HAP1 activator. However, it has no effect on the transcriptional activities of the human glucocorticoid receptor (hGR) and GCN4. The SIN3 protein contains four copies of a paired amphipathic helix (PAH) motif. Deletion analysis of the SIN3 PAH motifs shows that the PAH3 motif is essential for SIN3-mediated regulation of hPR, GAL4, and the HAPI activator. In constrast, the PAH 1, PAH2, and PAH4 motifs are not required for SIN3-mediated regulation of these activators. Additionally, we examined the mechanism(s) by which the SIN3 protein modulate the activities of various activators. We are unable to demonstrate the direct interaction of SIN3 protein with these activators using the yeast two-hybrid system or co-immunoprecipitation. These data suggest that SIN3 regulates the transactivation functions of hPR, GAL4, and the HAP1 activator by an indirect mechanism.

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