The yeast adaptor protein complex, AP-3, is essential for the efficient delivery of alkaline phosphatase by the alternate pathway to the vacuole

J. David Stepp, Kristen Huang, Sandra Lemmon

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

A novel clathrin adaptor-like complex, adaptor protein (AP)-3, has recently been described in yeast and in animals. To gain insight into the role of yeast AP-3, a genetic strategy was devised to isolate gene products that are required in the absence of the AP-3 μ chain encoded by APM3. One gene identified by this synthetic lethal screen was VPS45. The Vps pathway defines the route that several proteins, including carboxypeptidase Y, take from the late Golgi to the vacuole. However, vacuolar alkaline phosphatase (ALP) is transported via an alternate, intracellular route. This suggested that the apm3-Δ vps45 synthetic phenotype could be caused by a block in both the alternate and the Vps pathways. Here we demonstrate that loss of function of the AP-3 complex results in slowed processing and missorting of ALP. ALP is no longer localized to the vacuole membrane by immunofluorescence, but is found in small punctate structures throughout the cell. This pattern is distinct from the Golgi marker Kex2p, which is unaffected in AP-3 mutants. We also show that in the apm3-Δ mutant some ALP is delivered to the vacuole by diversion into the Vps pathway. Class E vps mutants accumulate an exaggerated prevacuolar compartment containing membrane proteins on their way to the vacuole or destined for recycling to the Golgi. Surprisingly, in AP-3 class E vps double mutants these proteins reappear on the vacuole. We suggest that some AP-3-dependent cargo proteins that regulate late steps in Golgi to vacuole transport are diverted into the Vps pathway allowing completion of transfer to the vacuole in the class E vps mutant.

Original languageEnglish
Pages (from-to)1761-1774
Number of pages14
JournalJournal of Cell Biology
Volume139
Issue number7
DOIs
StatePublished - Dec 29 1997
Externally publishedYes

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Adaptor Protein Complex 3
Fungal Proteins
Vacuoles
Alkaline Phosphatase
Mutant Proteins
Proteins
Vesicular Transport Adaptor Proteins
Cathepsin A
Synthetic Genes
Recycling
Fluorescent Antibody Technique
Membrane Proteins
Yeasts
Phenotype

ASJC Scopus subject areas

  • Cell Biology

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The yeast adaptor protein complex, AP-3, is essential for the efficient delivery of alkaline phosphatase by the alternate pathway to the vacuole. / Stepp, J. David; Huang, Kristen; Lemmon, Sandra.

In: Journal of Cell Biology, Vol. 139, No. 7, 29.12.1997, p. 1761-1774.

Research output: Contribution to journalArticle

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