The x gene is essential for HTLV replication

I. S Y Chen, D. J. Slamon, Joseph D Rosenblatt, N. P. Shah, S. G. Quan, W. Wachsman

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The human T-cell leukemia viruses (HTLV) are associated with T-cell malignancies in man and will transform normal human T cells in vitro. The mechanism of malignant transformation by HTLV is unknown but appears to be distinct from that of other classes of retroviruses, which induce malignant transformation through viral or cellular oncogenes. Recently a new gene, termed x, was identified in HTLV. This gene has been hypothesized to be the transforming gene of HTLV because of its conservation within the HTLV class of retroviruses. By in vitro mutagenesis of the HTLV-II x gene, it is now demonstrated that the presence of a functional x gene product is necessary for efficient HTLV transcription. Therefore, these studies provide direct evidence for an important function of the x gene in HTLV replication. The functional analogies between the x gene and transcriptional regulatory genes of some DNA viruses suggest that these viruses share similar mechanisms for cellular transformation.

Original languageEnglish
Pages (from-to)54-58
Number of pages5
JournalScience
Volume229
Issue number4708
DOIs
StatePublished - Nov 13 1985
Externally publishedYes

Fingerprint

Deltaretrovirus
Essential Genes
Virus Replication
Genes
Retroviridae
Oncogenes
Human T-lymphotropic virus 2
T-Lymphocytes
DNA Viruses
Regulator Genes
Mutagenesis
Viruses

ASJC Scopus subject areas

  • General

Cite this

Chen, I. S. Y., Slamon, D. J., Rosenblatt, J. D., Shah, N. P., Quan, S. G., & Wachsman, W. (1985). The x gene is essential for HTLV replication. Science, 229(4708), 54-58. https://doi.org/10.1126/science.2990037

The x gene is essential for HTLV replication. / Chen, I. S Y; Slamon, D. J.; Rosenblatt, Joseph D; Shah, N. P.; Quan, S. G.; Wachsman, W.

In: Science, Vol. 229, No. 4708, 13.11.1985, p. 54-58.

Research output: Contribution to journalArticle

Chen, ISY, Slamon, DJ, Rosenblatt, JD, Shah, NP, Quan, SG & Wachsman, W 1985, 'The x gene is essential for HTLV replication', Science, vol. 229, no. 4708, pp. 54-58. https://doi.org/10.1126/science.2990037
Chen ISY, Slamon DJ, Rosenblatt JD, Shah NP, Quan SG, Wachsman W. The x gene is essential for HTLV replication. Science. 1985 Nov 13;229(4708):54-58. https://doi.org/10.1126/science.2990037
Chen, I. S Y ; Slamon, D. J. ; Rosenblatt, Joseph D ; Shah, N. P. ; Quan, S. G. ; Wachsman, W. / The x gene is essential for HTLV replication. In: Science. 1985 ; Vol. 229, No. 4708. pp. 54-58.
@article{e5c9d3bc08c64cef8d0ee87137414d41,
title = "The x gene is essential for HTLV replication",
abstract = "The human T-cell leukemia viruses (HTLV) are associated with T-cell malignancies in man and will transform normal human T cells in vitro. The mechanism of malignant transformation by HTLV is unknown but appears to be distinct from that of other classes of retroviruses, which induce malignant transformation through viral or cellular oncogenes. Recently a new gene, termed x, was identified in HTLV. This gene has been hypothesized to be the transforming gene of HTLV because of its conservation within the HTLV class of retroviruses. By in vitro mutagenesis of the HTLV-II x gene, it is now demonstrated that the presence of a functional x gene product is necessary for efficient HTLV transcription. Therefore, these studies provide direct evidence for an important function of the x gene in HTLV replication. The functional analogies between the x gene and transcriptional regulatory genes of some DNA viruses suggest that these viruses share similar mechanisms for cellular transformation.",
author = "Chen, {I. S Y} and Slamon, {D. J.} and Rosenblatt, {Joseph D} and Shah, {N. P.} and Quan, {S. G.} and W. Wachsman",
year = "1985",
month = "11",
day = "13",
doi = "10.1126/science.2990037",
language = "English",
volume = "229",
pages = "54--58",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "4708",

}

TY - JOUR

T1 - The x gene is essential for HTLV replication

AU - Chen, I. S Y

AU - Slamon, D. J.

AU - Rosenblatt, Joseph D

AU - Shah, N. P.

AU - Quan, S. G.

AU - Wachsman, W.

PY - 1985/11/13

Y1 - 1985/11/13

N2 - The human T-cell leukemia viruses (HTLV) are associated with T-cell malignancies in man and will transform normal human T cells in vitro. The mechanism of malignant transformation by HTLV is unknown but appears to be distinct from that of other classes of retroviruses, which induce malignant transformation through viral or cellular oncogenes. Recently a new gene, termed x, was identified in HTLV. This gene has been hypothesized to be the transforming gene of HTLV because of its conservation within the HTLV class of retroviruses. By in vitro mutagenesis of the HTLV-II x gene, it is now demonstrated that the presence of a functional x gene product is necessary for efficient HTLV transcription. Therefore, these studies provide direct evidence for an important function of the x gene in HTLV replication. The functional analogies between the x gene and transcriptional regulatory genes of some DNA viruses suggest that these viruses share similar mechanisms for cellular transformation.

AB - The human T-cell leukemia viruses (HTLV) are associated with T-cell malignancies in man and will transform normal human T cells in vitro. The mechanism of malignant transformation by HTLV is unknown but appears to be distinct from that of other classes of retroviruses, which induce malignant transformation through viral or cellular oncogenes. Recently a new gene, termed x, was identified in HTLV. This gene has been hypothesized to be the transforming gene of HTLV because of its conservation within the HTLV class of retroviruses. By in vitro mutagenesis of the HTLV-II x gene, it is now demonstrated that the presence of a functional x gene product is necessary for efficient HTLV transcription. Therefore, these studies provide direct evidence for an important function of the x gene in HTLV replication. The functional analogies between the x gene and transcriptional regulatory genes of some DNA viruses suggest that these viruses share similar mechanisms for cellular transformation.

UR - http://www.scopus.com/inward/record.url?scp=0021879851&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021879851&partnerID=8YFLogxK

U2 - 10.1126/science.2990037

DO - 10.1126/science.2990037

M3 - Article

C2 - 2990037

AN - SCOPUS:0021879851

VL - 229

SP - 54

EP - 58

JO - Science

JF - Science

SN - 0036-8075

IS - 4708

ER -