The utility of 3D ultramicroscopy for evaluating cellular therapies after spinal cord injury

M. Ghosh, N. Jährling, M. C. Henao, H. U. Dodt, D. D. Pearse

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Cell therapies have shown promise for repairing the injured spinal cord in experimental models and are now being evaluated in clinical trials for the treatment of human spinal cord injury (SCI). To date, experimental evaluation of implanted cell survival, migration, and integration within the injured central nervous system (CNS) of animals has been technically demanding, requiring tissue sectioning, staining, imaging, and manual reconstruction of 2-dimensional (2D) specimens in 3 dimensions (3D). Not only are these histological procedures laborious and fraught with processing artifacts during manual 3D reconstruction, but they are time-intensive. Herein we describe the utility of 3D ultramicroscopy for assessment of cell therapies after SCI, a new state-of-the-art imaging modality in which whole brain and spinal cord samples are optically sectioned to allow evaluation of intact, macroscopic specimens with microscopic resolution.

Original languageEnglish (US)
Pages (from-to)73-76
Number of pages4
JournalTopics in Spinal Cord Injury Rehabilitation
Volume18
Issue number1
DOIs
StatePublished - Jan 1 2012

Keywords

  • 3D ultramicroscopy
  • cell therapy
  • Schwann cells
  • spinal cord injury

ASJC Scopus subject areas

  • Clinical Neurology
  • Rehabilitation
  • Physical Therapy, Sports Therapy and Rehabilitation

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