Abstract
A neuronal culture system has been developed that has demonstrated to induce myelin formation by added oligodendrocytes. Networks of dissociated dorsal root ganglion neurons were prepared by suppressing non-neuronal cells (i.e. fibroblasts and Schwann cells) with a continuous 2 week exposure to 10-5 M fluorodeoxyuridine in the culture medium. After drug withdrawal, neuroglial cells were introduced in optic nerve implants from 1-2 week-old rats. These added glial cells migrated extensively over the unensheathed neurities and central myelin was formed by 2 weeks after the implant addition.
Original language | English (US) |
---|---|
Pages (from-to) | 247-252 |
Number of pages | 6 |
Journal | Brain Research |
Volume | 196 |
Issue number | 1 |
DOIs | |
State | Published - Aug 25 1980 |
Keywords
- CNS myelination
- neuroglial tissue culture
- oligodendrocytes
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)