The use of networks of dissociated rat dorsal root ganglion neurons to induce myelination by oligodendrocytes in culture

P. Wood; E. Okada; R. Bunge

doi:10.1016/0006-8993(80)90732-5

The use of networks of dissociated rat dorsal root ganglion neurons to induce myelination by oligodendrocytes in culture

P. Wood, E. Okada, R. Bunge

Neurological Surgery

Research output: Contribution to journal › Article

44 Scopus citations

Abstract

A neuronal culture system has been developed that has demonstrated to induce myelin formation by added oligodendrocytes. Networks of dissociated dorsal root ganglion neurons were prepared by suppressing non-neuronal cells (i.e. fibroblasts and Schwann cells) with a continuous 2 week exposure to 10^-5 M fluorodeoxyuridine in the culture medium. After drug withdrawal, neuroglial cells were introduced in optic nerve implants from 1-2 week-old rats. These added glial cells migrated extensively over the unensheathed neurities and central myelin was formed by 2 weeks after the implant addition.

Original language	English (US)
Pages (from-to)	247-252
Number of pages	6
Journal	Brain Research
Volume	196
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(80)90732-5
State	Published - Aug 25 1980

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Keywords

CNS myelination
neuroglial tissue culture
oligodendrocytes

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
Neuroscience(all)

Cite this

Wood, P., Okada, E., & Bunge, R. (1980). The use of networks of dissociated rat dorsal root ganglion neurons to induce myelination by oligodendrocytes in culture. Brain Research, 196(1), 247-252. https://doi.org/10.1016/0006-8993(80)90732-5

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10.1016/0006-8993(80)90732-5