The use of networks of dissociated rat dorsal root ganglion neurons to induce myelination by oligodendrocytes in culture

P. Wood, E. Okada, R. Bunge

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

A neuronal culture system has been developed that has demonstrated to induce myelin formation by added oligodendrocytes. Networks of dissociated dorsal root ganglion neurons were prepared by suppressing non-neuronal cells (i.e. fibroblasts and Schwann cells) with a continuous 2 week exposure to 10-5 M fluorodeoxyuridine in the culture medium. After drug withdrawal, neuroglial cells were introduced in optic nerve implants from 1-2 week-old rats. These added glial cells migrated extensively over the unensheathed neurities and central myelin was formed by 2 weeks after the implant addition.

Original languageEnglish (US)
Pages (from-to)247-252
Number of pages6
JournalBrain Research
Volume196
Issue number1
DOIs
StatePublished - Aug 25 1980

Keywords

  • CNS myelination
  • neuroglial tissue culture
  • oligodendrocytes

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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