The use of multiparametric monitoring during islet cell isolation and culture: A potential tool for in-process corrections of critical physiological factors

Chris Fraker, Jorge Montelongo, Joel Szust, Aisha Khan, Camillo Ricordi

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Variables such as pH, pCO2, and pO2 have been established in the literature as critical factors that could affect the outcome of the islet cell processing and, therefore, the quality of the cells that could be transplanted. This report describes a highly accurate continuous multiparametric monitoring system and its evaluation for continuous monitoring of physiological variables during critical steps of the islet isolation procedure as well as during in vitro culture of the insulin-producing cells. Close monitoring of these variables could be of assistance to improve the outcome of islet cell processing, allowing to identify as soon as possible problems that could be corrected during the procedure, as well as during in vitro preservation, or shipment to remote sites.

Original languageEnglish (US)
Pages (from-to)497-502
Number of pages6
JournalCell transplantation
Volume13
Issue number5
DOIs
StatePublished - 2004

Keywords

  • Assessment
  • Cell culture
  • Islet
  • Isolation
  • Monitoring
  • Optical probes

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

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