The use of keratin biomaterials derived from human hair for the promotion of rapid regeneration of peripheral nerves

Paulina Sierpinski, Jeffrey Garrett, Jianjun Ma, Peter Apel, David Klorig, Thomas Smith, L. Andrew Koman, Anthony Atala, Mark Van Dyke

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

The management of trauma-associated nerve defects is difficult because of the absence of autologous donor motor or sensory nerves. Pre-clinical development and clinical experience has shown that damaged nerves can be surgically repaired using a tubular conduit interposed across the defect. Acceptable patient outcomes are achieved so long as the gap distance does not exceed a few centimeters. Although research in animals has demonstrated that nerve repair can be facilitated across slightly larger gaps by introducing a biomaterial filler into the conduit lumen, these biomaterials are not typically "neuroinductive" (i.e. capable of acting directly on regenerative cells to enhance nerve tissue formation beyond clinical limits). Moreover, their use does not often result in functional recovery equivalent to nerve autograft, the clinical gold standard. Here we show that a biomaterial gel made from the proteins found in human hair can mediate a robust nerve regeneration response, in part through activation of Schwann cells. In vitro, keratins extracted from human hair enhance the activity of Schwann cells by a chemotactic mechanism, increase their attachment and proliferation, and up-regulate expression of important genes. Moreover, these characteristics translate to improved functional nerve recovery in an animal model. These results suggest that a biomaterial derived from human hair keratins is neuroinductive and can facilitate an outcome comparable to autograft in a nerve injury model.

Original languageEnglish
Pages (from-to)118-128
Number of pages11
JournalBiomaterials
Volume29
Issue number1
DOIs
StatePublished - Jan 1 2008
Externally publishedYes

Fingerprint

Keratin
Biocompatible Materials
Keratins
Peripheral Nerves
Biomaterials
Hair
Regeneration
Schwann Cells
Autografts
Animals
Hair-Specific Keratins
Cells
Recovery
Nerve Tissue
Defects
Nerve Regeneration
Wounds and Injuries
Fillers
Repair
Up-Regulation

Keywords

  • ECM
  • Keratin
  • Nerve guide
  • Nerve regeneration
  • Schwann cell

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

The use of keratin biomaterials derived from human hair for the promotion of rapid regeneration of peripheral nerves. / Sierpinski, Paulina; Garrett, Jeffrey; Ma, Jianjun; Apel, Peter; Klorig, David; Smith, Thomas; Koman, L. Andrew; Atala, Anthony; Van Dyke, Mark.

In: Biomaterials, Vol. 29, No. 1, 01.01.2008, p. 118-128.

Research output: Contribution to journalArticle

Sierpinski, P, Garrett, J, Ma, J, Apel, P, Klorig, D, Smith, T, Koman, LA, Atala, A & Van Dyke, M 2008, 'The use of keratin biomaterials derived from human hair for the promotion of rapid regeneration of peripheral nerves', Biomaterials, vol. 29, no. 1, pp. 118-128. https://doi.org/10.1016/j.biomaterials.2007.08.023
Sierpinski, Paulina ; Garrett, Jeffrey ; Ma, Jianjun ; Apel, Peter ; Klorig, David ; Smith, Thomas ; Koman, L. Andrew ; Atala, Anthony ; Van Dyke, Mark. / The use of keratin biomaterials derived from human hair for the promotion of rapid regeneration of peripheral nerves. In: Biomaterials. 2008 ; Vol. 29, No. 1. pp. 118-128.
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