The use of bromodeoxyuridine incorporation assays to assess corneal stem cell proliferation

Ashley M. Crane, Sanjoy K. Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations

Abstract

Bromodeoxyuridine (BrdU) incorporation assays have long been used to detect DNA synthesis in vivo and in vitro. The key principle of this method is that BrdU incorporated as a thymidine analog into nuclear DNA represents a label that can be tracked using antibody probes. In this chapter, we describe BrdU incorporation into limbal stem cells. The colorimetric reaction produced by this assay can be detected by immunohistochemistry, and using appropriate controls, it can be used for determination of proliferating properties of restricted progenitor cells derived from the cornea.

Original languageEnglish (US)
Title of host publicationCorneal Regenerative Medicine
Subtitle of host publicationMethods and Protocols
Pages65-70
Number of pages6
DOIs
StatePublished - Sep 4 2013

Publication series

NameMethods in Molecular Biology
Volume1014
ISSN (Print)1064-3745

Keywords

  • 5-Bromo-2′-deoxyuridine
  • Bromodeoxyuridine
  • Bromodeoxyuridine incorporation assay
  • Thymidine analog

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'The use of bromodeoxyuridine incorporation assays to assess corneal stem cell proliferation'. Together they form a unique fingerprint.

  • Cite this

    Crane, A. M., & Bhattacharya, S. K. (2013). The use of bromodeoxyuridine incorporation assays to assess corneal stem cell proliferation. In Corneal Regenerative Medicine: Methods and Protocols (pp. 65-70). (Methods in Molecular Biology; Vol. 1014). https://doi.org/10.1007/978-1-62703-432-6-4