The use of bromodeoxyuridine incorporation assays to assess corneal stem cell proliferation

Ashley M. Crane, Sanjoy K Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Citations (Scopus)

Abstract

Bromodeoxyuridine (BrdU) incorporation assays have long been used to detect DNA synthesis in vivo and in vitro. The key principle of this method is that BrdU incorporated as a thymidine analog into nuclear DNA represents a label that can be tracked using antibody probes. In this chapter, we describe BrdU incorporation into limbal stem cells. The colorimetric reaction produced by this assay can be detected by immunohistochemistry, and using appropriate controls, it can be used for determination of proliferating properties of restricted progenitor cells derived from the cornea.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages65-70
Number of pages6
Volume1014
DOIs
StatePublished - Sep 4 2013

Publication series

NameMethods in Molecular Biology
Volume1014
ISSN (Print)10643745

Fingerprint

Bromodeoxyuridine
Stem Cells
Cell Proliferation
DNA
Cornea
Thymidine
Immunohistochemistry
Antibodies

Keywords

  • 5-Bromo-2′-deoxyuridine
  • Bromodeoxyuridine
  • Bromodeoxyuridine incorporation assay
  • Thymidine analog

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Crane, A. M., & Bhattacharya, S. K. (2013). The use of bromodeoxyuridine incorporation assays to assess corneal stem cell proliferation. In Methods in Molecular Biology (Vol. 1014, pp. 65-70). (Methods in Molecular Biology; Vol. 1014). https://doi.org/10.1007/978-1-62703-432-6-4

The use of bromodeoxyuridine incorporation assays to assess corneal stem cell proliferation. / Crane, Ashley M.; Bhattacharya, Sanjoy K.

Methods in Molecular Biology. Vol. 1014 2013. p. 65-70 (Methods in Molecular Biology; Vol. 1014).

Research output: Chapter in Book/Report/Conference proceedingChapter

Crane, AM & Bhattacharya, SK 2013, The use of bromodeoxyuridine incorporation assays to assess corneal stem cell proliferation. in Methods in Molecular Biology. vol. 1014, Methods in Molecular Biology, vol. 1014, pp. 65-70. https://doi.org/10.1007/978-1-62703-432-6-4
Crane AM, Bhattacharya SK. The use of bromodeoxyuridine incorporation assays to assess corneal stem cell proliferation. In Methods in Molecular Biology. Vol. 1014. 2013. p. 65-70. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-432-6-4
Crane, Ashley M. ; Bhattacharya, Sanjoy K. / The use of bromodeoxyuridine incorporation assays to assess corneal stem cell proliferation. Methods in Molecular Biology. Vol. 1014 2013. pp. 65-70 (Methods in Molecular Biology).
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