Abstract
During the development of neural networks, what sets synaptogenic interactions apart from nonsynaptogenic interactions is not well understood at the subcellular level. Using a combination of intracellular dye injection and electron microscopy, we show that a specific motoneuron (RP3) and its synaptic partners (muscles 6 and 7), both often bearing micro-processes, develop intimate membrane contact sites characterized by junctional structures, prior to their initiating synaptogenesis in Drosophila embryos. Other motoneuron growth cones that extend alongside the RP3 growth cone to innervate surrounding muscles do not form such contacts with muscles 6 and 7. We also examined how specific target recognition molecules affect the development of these ultrastructural associations between synaptic partner cells. When Fasciclin III (Fas3), a 'positive' target recognition molecule for RP3, is ectopically expressed in neighboring muscles, the RP3 growth cone ectopically develops membrane contact sites with Fas3-misexpressing muscles with which it would not normally associate. In contrast, when Toll, a 'negative' target recognition molecule normally expressed by a subset of muscles that surrounds muscles 6 and 7, is misexpressed on muscles 6 and 7, the RP3 growth cone fails to exhibit its normal close contact with these muscles. We propose that the formation of close membrane associations and junctional structures can be regulated under the influence of synaptic target recognition molecules and signifies the beginning of subcellular events during synaptic target recognition. (C) 2000 John Wiley and Sons, Inc.
| Original language | English |
|---|---|
| Pages (from-to) | 448-459 |
| Number of pages | 12 |
| Journal | Journal of Neurobiology |
| Volume | 42 |
| Issue number | 4 |
| DOIs | |
| State | Published - Mar 13 2000 |
| Externally published | Yes |
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Keywords
- Fasciclin III
- Growth cone
- Microprocess
- Synaptogenesis
- Toll
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
The ultrastructural interactions of identified pre- and postsynaptic cells during synaptic target recognition in Drosophila embryos. / Suzuki, Emiko; Rose, Demian; Chiba, Akira.
In: Journal of Neurobiology, Vol. 42, No. 4, 13.03.2000, p. 448-459.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The ultrastructural interactions of identified pre- and postsynaptic cells during synaptic target recognition in Drosophila embryos
AU - Suzuki, Emiko
AU - Rose, Demian
AU - Chiba, Akira
PY - 2000/3/13
Y1 - 2000/3/13
N2 - During the development of neural networks, what sets synaptogenic interactions apart from nonsynaptogenic interactions is not well understood at the subcellular level. Using a combination of intracellular dye injection and electron microscopy, we show that a specific motoneuron (RP3) and its synaptic partners (muscles 6 and 7), both often bearing micro-processes, develop intimate membrane contact sites characterized by junctional structures, prior to their initiating synaptogenesis in Drosophila embryos. Other motoneuron growth cones that extend alongside the RP3 growth cone to innervate surrounding muscles do not form such contacts with muscles 6 and 7. We also examined how specific target recognition molecules affect the development of these ultrastructural associations between synaptic partner cells. When Fasciclin III (Fas3), a 'positive' target recognition molecule for RP3, is ectopically expressed in neighboring muscles, the RP3 growth cone ectopically develops membrane contact sites with Fas3-misexpressing muscles with which it would not normally associate. In contrast, when Toll, a 'negative' target recognition molecule normally expressed by a subset of muscles that surrounds muscles 6 and 7, is misexpressed on muscles 6 and 7, the RP3 growth cone fails to exhibit its normal close contact with these muscles. We propose that the formation of close membrane associations and junctional structures can be regulated under the influence of synaptic target recognition molecules and signifies the beginning of subcellular events during synaptic target recognition. (C) 2000 John Wiley and Sons, Inc.
AB - During the development of neural networks, what sets synaptogenic interactions apart from nonsynaptogenic interactions is not well understood at the subcellular level. Using a combination of intracellular dye injection and electron microscopy, we show that a specific motoneuron (RP3) and its synaptic partners (muscles 6 and 7), both often bearing micro-processes, develop intimate membrane contact sites characterized by junctional structures, prior to their initiating synaptogenesis in Drosophila embryos. Other motoneuron growth cones that extend alongside the RP3 growth cone to innervate surrounding muscles do not form such contacts with muscles 6 and 7. We also examined how specific target recognition molecules affect the development of these ultrastructural associations between synaptic partner cells. When Fasciclin III (Fas3), a 'positive' target recognition molecule for RP3, is ectopically expressed in neighboring muscles, the RP3 growth cone ectopically develops membrane contact sites with Fas3-misexpressing muscles with which it would not normally associate. In contrast, when Toll, a 'negative' target recognition molecule normally expressed by a subset of muscles that surrounds muscles 6 and 7, is misexpressed on muscles 6 and 7, the RP3 growth cone fails to exhibit its normal close contact with these muscles. We propose that the formation of close membrane associations and junctional structures can be regulated under the influence of synaptic target recognition molecules and signifies the beginning of subcellular events during synaptic target recognition. (C) 2000 John Wiley and Sons, Inc.
KW - Fasciclin III
KW - Growth cone
KW - Microprocess
KW - Synaptogenesis
KW - Toll
UR - http://www.scopus.com/inward/record.url?scp=0034053085&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034053085&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-4695(200003)42:4<448::AID-NEU6>3.0.CO;2-H
DO - 10.1002/(SICI)1097-4695(200003)42:4<448::AID-NEU6>3.0.CO;2-H
M3 - Article
C2 - 10699982
AN - SCOPUS:0034053085
VL - 42
SP - 448
EP - 459
JO - Developmental Neurobiology
JF - Developmental Neurobiology
SN - 0022-3034
IS - 4
ER -