The ubiquitin-conjugating enzyme UBCH7 acts as a coactivator for steroid hormone receptors

Seema Verma, Ayesha Ismail, Xiuhua Gao, Guilian Fu, Xiaotao Li, Bert W. O'Malley, Zafar Nawaz

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

We investigated the role of the ubiquitin-conjugating enzyme UBCH7 in nuclear receptor transactivation. Using transient transfection assays, we demonstrated that UBCH7 modulates the transcriptional activity of progesterone receptor (PR) and glucocorticoid, androgen, and retinoic acid receptors in a hormone-dependent manner and that the ubiquitin conjugation activity of UBCH7 is required for its ability to potentiate transactivation by steroid hormone receptors (SHR). However, UBCH7 showed no significant effect on the transactivation functions of p53 and VP-16 activation domain. Depletion of endogenous UBCH7 protein by small interfering RNAs suggests that UBCH7 is required for the proper function of SHR. Furthermore, a chromatin immunoprecipitation assay demonstrated the hormone-dependent recruitment of UBCH7 onto estrogen receptor- and PR-responsive promoters. Additionally, we show that UBCH7 and E6-associated protein (E6-AP) synergistically enhance PR transactivation. We also demonstrate that UBCH7 interacts with steroid receptor coactivator 1 (SRC-1) and that UBCH7 coactivation function is dependent on SRC-1. Taken together, our results reveal the possible role of UBCH7 in steroid receptor transactivation and provide insights into the mechanism of action of UBCH7 in receptor function.

Original languageEnglish
Pages (from-to)8716-8726
Number of pages11
JournalMolecular and Cellular Biology
Volume24
Issue number19
DOIs
StatePublished - Oct 1 2004
Externally publishedYes

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Ubiquitin-Conjugating Enzymes
Steroid Receptors
Transcriptional Activation
Hormones
Progesterone Receptors
Nuclear Receptor Coactivator 1
Retinoic Acid Receptors
Chromatin Immunoprecipitation
Etoposide
Cytoplasmic and Nuclear Receptors
Ubiquitin
Estrogen Receptors
Small Interfering RNA
Glucocorticoids
Androgens
Transfection
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The ubiquitin-conjugating enzyme UBCH7 acts as a coactivator for steroid hormone receptors. / Verma, Seema; Ismail, Ayesha; Gao, Xiuhua; Fu, Guilian; Li, Xiaotao; O'Malley, Bert W.; Nawaz, Zafar.

In: Molecular and Cellular Biology, Vol. 24, No. 19, 01.10.2004, p. 8716-8726.

Research output: Contribution to journalArticle

Verma, Seema ; Ismail, Ayesha ; Gao, Xiuhua ; Fu, Guilian ; Li, Xiaotao ; O'Malley, Bert W. ; Nawaz, Zafar. / The ubiquitin-conjugating enzyme UBCH7 acts as a coactivator for steroid hormone receptors. In: Molecular and Cellular Biology. 2004 ; Vol. 24, No. 19. pp. 8716-8726.
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