The transforming proteins of PRCII virus and Rous sarcoma virus form a complex with the same two cellular phosphoproteins

Rebecca D Adkins, T. Hunter, B. M. Sefton

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

P105 and P110, the presumptive transforming proteins of PRCII avian sarcoma virus, have been found to be present in transformed chicken cells in two forms: as monomers and as part of a complex which contains both a 50,000-dalton and a 90,000-dalton cellular phosphoprotein. The 90,000-dalton cellular protein was found to be identical to one of the proteins in chicken cells whose synthesis is induced by stress. The 50,000-dalton protein was found to contain phosphotyrosine when isolated from the complex and therefore may be a substrate for the tyrosine protein kinase activity which is associated with P105 and P110. These same two cellular phosphoproteins have previously been shown to be present in a complex with pp60(src), the tyrosine protein kinase which is the transforming protein of Rous sarcoma virus. However, not all avian sarcoma virus transforming proteins with associated tyrosine protein kinase activities form a complex efficiently with these cellular proteins. Little if any of P90, the putative transforming protein of Yamaguchi 73 virus, was found in a complex with the 50,000-dalton and 90,000-dalton cellular phosphoproteins.

Original languageEnglish
Pages (from-to)448-455
Number of pages8
JournalJournal of Virology
Volume43
Issue number2
StatePublished - Jan 1 1982
Externally publishedYes

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Rous sarcoma virus
phosphoproteins
Phosphoproteins
Viruses
viruses
Oncogene Protein pp60(v-src)
protein-tyrosine kinases
Proteins
proteins
Protein-Tyrosine Kinases
Chickens
Phosphotyrosine
src-Family Kinases
chickens
cells
synthesis

ASJC Scopus subject areas

  • Immunology

Cite this

The transforming proteins of PRCII virus and Rous sarcoma virus form a complex with the same two cellular phosphoproteins. / Adkins, Rebecca D; Hunter, T.; Sefton, B. M.

In: Journal of Virology, Vol. 43, No. 2, 01.01.1982, p. 448-455.

Research output: Contribution to journalArticle

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AB - P105 and P110, the presumptive transforming proteins of PRCII avian sarcoma virus, have been found to be present in transformed chicken cells in two forms: as monomers and as part of a complex which contains both a 50,000-dalton and a 90,000-dalton cellular phosphoprotein. The 90,000-dalton cellular protein was found to be identical to one of the proteins in chicken cells whose synthesis is induced by stress. The 50,000-dalton protein was found to contain phosphotyrosine when isolated from the complex and therefore may be a substrate for the tyrosine protein kinase activity which is associated with P105 and P110. These same two cellular phosphoproteins have previously been shown to be present in a complex with pp60(src), the tyrosine protein kinase which is the transforming protein of Rous sarcoma virus. However, not all avian sarcoma virus transforming proteins with associated tyrosine protein kinase activities form a complex efficiently with these cellular proteins. Little if any of P90, the putative transforming protein of Yamaguchi 73 virus, was found in a complex with the 50,000-dalton and 90,000-dalton cellular phosphoproteins.

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