@article{edadadb2da8146fd829dcf8ec87dea62,
title = "The transcriptome of the early life history stages of the California sea hare aplysia Californica",
abstract = "Aplysia californica is a marine opisthobranch mollusc used as a model organism in neurobiology for cellular analyses of learning and behavior because it possesses a comparatively small number of neurons of large size. The mollusca comprise the second largest animal phylum, yet detailed genetic and genomic information is only recently beginning to accrue. Thus developmental and comparative evolutionary biology as well as biomedical research would benefit from additional information on DNA sequences of Aplysia. Therefore, we have constructed a series of unidirectional cDNA libraries from different life stages of Aplysia. These include whole organisms from the egg, veliger, metamorphic, and juvenile stages as well as adult neural tissue for reference. Individual clones were randomly picked, and high-throughput, single pass sequence analysis was performed to generate 7971 sequences. Of these, there were 5507 quality-filtered ESTs that clustered into 1988 unigenes, which are annotated and deposited into GenBank. A significant number (497) of ESTs did not match existing Aplysia ESTs and are thus potentially novel sequences for Aplysia. GO and KEGG analyses of these novel sequences indicated that a large number were involved in protein binding and translation, consistent with the predominant biosynthetic role in development and the presence of stage-specific protein isoforms.",
keywords = "Aplysia, cDNA libraries, Developmental stages, ESTs, Genomics, Veliger",
author = "Fiedler, {T. J.} and A. Hudder and McKay, {S. J.} and S. Shivkumar and Capo, {T. R.} and Schmale, {M. C.} and Walsh, {P. J.}",
note = "Funding Information: All developmental stages of A. californica were obtained from the National Resource for Aplysia at the University of Miami, supported by the National Center for Research Resources of the U.S. National Institutes of Health. The developmental phases and the stages within each phase used in this study adhere to the nomenclature of Kriegstein (1977) and were: (1) EGGS consisting of fertilized egg strands collected each day from the date laid through pre-hatching, encompassing all embryonic stages. After RNA extraction (see below), the samples were pooled; (2) VELI consists of free-swimming veliger larvae, stages 1–5 veliger larvae; (3) JMAC consists of metamorphosing larvae, stages 6–8 in transition from veliger to settled juveniles; (4) JUVE is made from pre-sexually mature settled juveniles, stages 9–11; and (5) ACAN is made from neural tissue only from adult animals of a range of ages from young adult through senescence, stages 12 and 13. The neural tissue includes all five major pairs of ganglia: buccal, cerebral, pleural, pedal and abdominal. Movies of each developmental phase can be accessed at http://aplysia.miami.edu/life-cycle.html . Funding Information: The authors wish to acknowledge NCRR grant P40 RR10294 to MCS, NSF grant ( OCE-0215667 ) to PJW, and a University of Miami Board of Trustees Innovation Fund award to PJW and MCS. AH also thanks the Korein Foundation for their generous donations to the project and Julia Radic for assistance in library construction. PJW is supported by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada, the Canada Research Chairs Program, and the Canada Foundation for Innovation. ",
year = "2010",
month = jun,
doi = "10.1016/j.cbd.2010.03.003",
language = "English (US)",
volume = "5",
pages = "165--170",
journal = "Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics",
issn = "1744-117X",
publisher = "Elsevier Inc.",
number = "2",
}