The transcriptome of HCV replicon expressing cell lines in the presence of alpha interferon

Junpei Hayashi, Radka Stoyanova, Christoph Seeger

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

We have used DNA microarray analysis of human hepatoma and epithelial carcinoma cells expressing hepatitis C virus (HCV) subgenomic replicons to test whether HCV replication alters gene expression and influences the alpha interferon (IFN-α) response. We directly compared the HCV replicon system with a similar system based on a subgenomic replicon of the West Nile virus (WNV) subtype Kunjin virus. We found that in contrast to WNV replicons, persistent replication of HCV replicons did not significantly alter the transcriptome of infected cells nor did it inhibit the nature of the IFN-stimulated genes (ISGs). Our results also provided evidence for the existence of a small number of ISGs that could play a role in the inhibition of HCV replication by IFN-α. Finally, we identified ISGs that are activated by the cytokine in a cell-type specific fashion.

Original languageEnglish (US)
Pages (from-to)264-275
Number of pages12
JournalVirology
Volume335
Issue number2
DOIs
StatePublished - May 10 2005
Externally publishedYes

Keywords

  • HCV replicon
  • Interferon
  • Transcriptome

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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