The toxicity of microcystin LR in mice following 7 days of inhalation exposure

Janet M. Benson, Julie A. Hutt, Kathleen Rein, Susan E. Boggs, Edward B. Barr, Lora E. Fleming

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Microcystins, a family of cyclic heptapeptides produced by the cyanobacteria, Microcystis aeruginosa, have documented hepatotoxic and tumor promoting activities. The purpose of this study was to evaluate the toxicity of inhaled microcystin LR (microcystin). Male BALB/c mice were exposed by nose-only inhalation to 260-265 μg microcystin/m3 for 7 days. The low-, mid- and high-dose groups were exposed for 0.5, 1, and 2 h, respectively. Control animals were sham exposed to aerosolized vehicle. Treatment-related microscopic lesions were observed only in the nasal cavity of the mid- and high-dose groups. These lesions consisted of minimal to moderate multifocal degeneration and necrosis of the respiratory epithelium, with variable neutrophilic inflammation and minimal to marked degeneration, necrosis, and atrophy of the olfactory epithelium. The no-adverse-effect dose for the nasal lesions was approximately 3 μg/kg body weight, or 20 ng/cm2 of nasal epithelium. In serum, only two protein peaks, occurring at m/zs of 11,688 and 11,829 Da, exhibited decreases in intensity that were microcystin dose-dependent. While these proteins have not been positively identified, they may be useful in the future as biomarkers of microcystin exposure in humans.

Original languageEnglish
Pages (from-to)691-698
Number of pages8
JournalToxicon
Volume45
Issue number6
DOIs
StatePublished - May 1 2005
Externally publishedYes

Fingerprint

Inhalation Exposure
Toxicity
Nose
Necrosis
Microcystins
Microcystis
Olfactory Mucosa
Respiratory Mucosa
Nasal Mucosa
Nasal Cavity
Cyanobacteria
Biomarkers
Inhalation
Atrophy
Tumors
Animals
Proteins
Body Weight
Inflammation
cyanoginosin LR

Keywords

  • Inhalation
  • Microcystin
  • Nasal epithelium
  • Protein expression in serum

ASJC Scopus subject areas

  • Toxicology

Cite this

Benson, J. M., Hutt, J. A., Rein, K., Boggs, S. E., Barr, E. B., & Fleming, L. E. (2005). The toxicity of microcystin LR in mice following 7 days of inhalation exposure. Toxicon, 45(6), 691-698. https://doi.org/10.1016/j.toxicon.2005.01.004

The toxicity of microcystin LR in mice following 7 days of inhalation exposure. / Benson, Janet M.; Hutt, Julie A.; Rein, Kathleen; Boggs, Susan E.; Barr, Edward B.; Fleming, Lora E.

In: Toxicon, Vol. 45, No. 6, 01.05.2005, p. 691-698.

Research output: Contribution to journalArticle

Benson, JM, Hutt, JA, Rein, K, Boggs, SE, Barr, EB & Fleming, LE 2005, 'The toxicity of microcystin LR in mice following 7 days of inhalation exposure', Toxicon, vol. 45, no. 6, pp. 691-698. https://doi.org/10.1016/j.toxicon.2005.01.004
Benson JM, Hutt JA, Rein K, Boggs SE, Barr EB, Fleming LE. The toxicity of microcystin LR in mice following 7 days of inhalation exposure. Toxicon. 2005 May 1;45(6):691-698. https://doi.org/10.1016/j.toxicon.2005.01.004
Benson, Janet M. ; Hutt, Julie A. ; Rein, Kathleen ; Boggs, Susan E. ; Barr, Edward B. ; Fleming, Lora E. / The toxicity of microcystin LR in mice following 7 days of inhalation exposure. In: Toxicon. 2005 ; Vol. 45, No. 6. pp. 691-698.
@article{2f497416298749fc985c46bbc4039da2,
title = "The toxicity of microcystin LR in mice following 7 days of inhalation exposure",
abstract = "Microcystins, a family of cyclic heptapeptides produced by the cyanobacteria, Microcystis aeruginosa, have documented hepatotoxic and tumor promoting activities. The purpose of this study was to evaluate the toxicity of inhaled microcystin LR (microcystin). Male BALB/c mice were exposed by nose-only inhalation to 260-265 μg microcystin/m3 for 7 days. The low-, mid- and high-dose groups were exposed for 0.5, 1, and 2 h, respectively. Control animals were sham exposed to aerosolized vehicle. Treatment-related microscopic lesions were observed only in the nasal cavity of the mid- and high-dose groups. These lesions consisted of minimal to moderate multifocal degeneration and necrosis of the respiratory epithelium, with variable neutrophilic inflammation and minimal to marked degeneration, necrosis, and atrophy of the olfactory epithelium. The no-adverse-effect dose for the nasal lesions was approximately 3 μg/kg body weight, or 20 ng/cm2 of nasal epithelium. In serum, only two protein peaks, occurring at m/zs of 11,688 and 11,829 Da, exhibited decreases in intensity that were microcystin dose-dependent. While these proteins have not been positively identified, they may be useful in the future as biomarkers of microcystin exposure in humans.",
keywords = "Inhalation, Microcystin, Nasal epithelium, Protein expression in serum",
author = "Benson, {Janet M.} and Hutt, {Julie A.} and Kathleen Rein and Boggs, {Susan E.} and Barr, {Edward B.} and Fleming, {Lora E.}",
year = "2005",
month = "5",
day = "1",
doi = "10.1016/j.toxicon.2005.01.004",
language = "English",
volume = "45",
pages = "691--698",
journal = "Toxicon",
issn = "0041-0101",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - The toxicity of microcystin LR in mice following 7 days of inhalation exposure

AU - Benson, Janet M.

AU - Hutt, Julie A.

AU - Rein, Kathleen

AU - Boggs, Susan E.

AU - Barr, Edward B.

AU - Fleming, Lora E.

PY - 2005/5/1

Y1 - 2005/5/1

N2 - Microcystins, a family of cyclic heptapeptides produced by the cyanobacteria, Microcystis aeruginosa, have documented hepatotoxic and tumor promoting activities. The purpose of this study was to evaluate the toxicity of inhaled microcystin LR (microcystin). Male BALB/c mice were exposed by nose-only inhalation to 260-265 μg microcystin/m3 for 7 days. The low-, mid- and high-dose groups were exposed for 0.5, 1, and 2 h, respectively. Control animals were sham exposed to aerosolized vehicle. Treatment-related microscopic lesions were observed only in the nasal cavity of the mid- and high-dose groups. These lesions consisted of minimal to moderate multifocal degeneration and necrosis of the respiratory epithelium, with variable neutrophilic inflammation and minimal to marked degeneration, necrosis, and atrophy of the olfactory epithelium. The no-adverse-effect dose for the nasal lesions was approximately 3 μg/kg body weight, or 20 ng/cm2 of nasal epithelium. In serum, only two protein peaks, occurring at m/zs of 11,688 and 11,829 Da, exhibited decreases in intensity that were microcystin dose-dependent. While these proteins have not been positively identified, they may be useful in the future as biomarkers of microcystin exposure in humans.

AB - Microcystins, a family of cyclic heptapeptides produced by the cyanobacteria, Microcystis aeruginosa, have documented hepatotoxic and tumor promoting activities. The purpose of this study was to evaluate the toxicity of inhaled microcystin LR (microcystin). Male BALB/c mice were exposed by nose-only inhalation to 260-265 μg microcystin/m3 for 7 days. The low-, mid- and high-dose groups were exposed for 0.5, 1, and 2 h, respectively. Control animals were sham exposed to aerosolized vehicle. Treatment-related microscopic lesions were observed only in the nasal cavity of the mid- and high-dose groups. These lesions consisted of minimal to moderate multifocal degeneration and necrosis of the respiratory epithelium, with variable neutrophilic inflammation and minimal to marked degeneration, necrosis, and atrophy of the olfactory epithelium. The no-adverse-effect dose for the nasal lesions was approximately 3 μg/kg body weight, or 20 ng/cm2 of nasal epithelium. In serum, only two protein peaks, occurring at m/zs of 11,688 and 11,829 Da, exhibited decreases in intensity that were microcystin dose-dependent. While these proteins have not been positively identified, they may be useful in the future as biomarkers of microcystin exposure in humans.

KW - Inhalation

KW - Microcystin

KW - Nasal epithelium

KW - Protein expression in serum

UR - http://www.scopus.com/inward/record.url?scp=15944381973&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15944381973&partnerID=8YFLogxK

U2 - 10.1016/j.toxicon.2005.01.004

DO - 10.1016/j.toxicon.2005.01.004

M3 - Article

VL - 45

SP - 691

EP - 698

JO - Toxicon

JF - Toxicon

SN - 0041-0101

IS - 6

ER -