The toadfish serotonin 2A (5-HT2A) receptor

Molecular characterization and its potential role in urea excretion

Edward M. Mager, Lea R. Medeiros, Anthony P. Lange, Danielle M Mcdonald

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Based on early pharmacological work, the serotonin 2A (5-HT2A) receptor subtype is believed to be involved in the regulation of toadfish pulsatile urea excretion. The goal of the following study was to characterize the toadfish 5-HT2A receptor at a molecular level, to determine the tissues in which this receptor is predominantly expressed and to further investigate the pharmacological specificity of toadfish pulsatile urea excretion by examining the effect of ketanserin, a 5-HT2A receptor antagonist, on resting rates of pulsatile urea excretion. The full-length toadfish 5-HT2A receptor encodes a 496 amino acid sequence and shares 57-80% sequence identity to 5-HT2A receptors of other organisms, with 100% conservation among important ligand-binding residues. Toadfish 5-HT2A receptor mRNA expression was highest in the swim bladder and gonad, followed by the whole brain. All other tissues tested (esophagus, stomach, anterior intestine, posterior intestine, rectum, liver, kidney, heart, muscle and gill) had mRNA expression levels that were significantly less than whole brain. Toadfish 5-HT2A receptor mRNA expression within the brain was highest in the hindbrain, telencephalon and midbrain/diencephalon regions. Treatment with the 5-HT2A receptor antagonist, ketanserin, resulted in a significant decrease in the pulsatile component of spontaneous urea excretion due to a reduction in urea pulse size with no significant change in pulse frequency. These results lend further support for the 5-HT2A receptor in the regulation of pulsatile urea excretion in toadfish.

Original languageEnglish
Pages (from-to)319-326
Number of pages8
JournalComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
Volume163
Issue number3-4
DOIs
StatePublished - Nov 1 2012

Fingerprint

Batrachoidiformes
Receptor, Serotonin, 5-HT2A
Urea
Serotonin
Serotonin 5-HT2 Receptor Antagonists
Ketanserin
Brain
Messenger RNA
Intestines
Pharmacology
Tissue
Diencephalon
Telencephalon
Rhombencephalon
Gonads
Mesencephalon
Rectum
Liver
Esophagus
Pulse

Keywords

  • Ammonia
  • Gill
  • Gulf toadfish
  • Nitrogen excretion
  • Opsanus beta
  • Swim bladder
  • TUT
  • Urea transport

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Physiology

Cite this

The toadfish serotonin 2A (5-HT2A) receptor : Molecular characterization and its potential role in urea excretion. / Mager, Edward M.; Medeiros, Lea R.; Lange, Anthony P.; Mcdonald, Danielle M.

In: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology, Vol. 163, No. 3-4, 01.11.2012, p. 319-326.

Research output: Contribution to journalArticle

@article{2a14c4f60f02436f95cde1da4201a025,
title = "The toadfish serotonin 2A (5-HT2A) receptor: Molecular characterization and its potential role in urea excretion",
abstract = "Based on early pharmacological work, the serotonin 2A (5-HT2A) receptor subtype is believed to be involved in the regulation of toadfish pulsatile urea excretion. The goal of the following study was to characterize the toadfish 5-HT2A receptor at a molecular level, to determine the tissues in which this receptor is predominantly expressed and to further investigate the pharmacological specificity of toadfish pulsatile urea excretion by examining the effect of ketanserin, a 5-HT2A receptor antagonist, on resting rates of pulsatile urea excretion. The full-length toadfish 5-HT2A receptor encodes a 496 amino acid sequence and shares 57-80{\%} sequence identity to 5-HT2A receptors of other organisms, with 100{\%} conservation among important ligand-binding residues. Toadfish 5-HT2A receptor mRNA expression was highest in the swim bladder and gonad, followed by the whole brain. All other tissues tested (esophagus, stomach, anterior intestine, posterior intestine, rectum, liver, kidney, heart, muscle and gill) had mRNA expression levels that were significantly less than whole brain. Toadfish 5-HT2A receptor mRNA expression within the brain was highest in the hindbrain, telencephalon and midbrain/diencephalon regions. Treatment with the 5-HT2A receptor antagonist, ketanserin, resulted in a significant decrease in the pulsatile component of spontaneous urea excretion due to a reduction in urea pulse size with no significant change in pulse frequency. These results lend further support for the 5-HT2A receptor in the regulation of pulsatile urea excretion in toadfish.",
keywords = "Ammonia, Gill, Gulf toadfish, Nitrogen excretion, Opsanus beta, Swim bladder, TUT, Urea transport",
author = "Mager, {Edward M.} and Medeiros, {Lea R.} and Lange, {Anthony P.} and Mcdonald, {Danielle M}",
year = "2012",
month = "11",
day = "1",
doi = "10.1016/j.cbpa.2012.07.013",
language = "English",
volume = "163",
pages = "319--326",
journal = "Comparative Biochemistry & Physiology; A: Comparative Physiology",
issn = "1095-6433",
publisher = "Elsevier Inc.",
number = "3-4",

}

TY - JOUR

T1 - The toadfish serotonin 2A (5-HT2A) receptor

T2 - Molecular characterization and its potential role in urea excretion

AU - Mager, Edward M.

AU - Medeiros, Lea R.

AU - Lange, Anthony P.

AU - Mcdonald, Danielle M

PY - 2012/11/1

Y1 - 2012/11/1

N2 - Based on early pharmacological work, the serotonin 2A (5-HT2A) receptor subtype is believed to be involved in the regulation of toadfish pulsatile urea excretion. The goal of the following study was to characterize the toadfish 5-HT2A receptor at a molecular level, to determine the tissues in which this receptor is predominantly expressed and to further investigate the pharmacological specificity of toadfish pulsatile urea excretion by examining the effect of ketanserin, a 5-HT2A receptor antagonist, on resting rates of pulsatile urea excretion. The full-length toadfish 5-HT2A receptor encodes a 496 amino acid sequence and shares 57-80% sequence identity to 5-HT2A receptors of other organisms, with 100% conservation among important ligand-binding residues. Toadfish 5-HT2A receptor mRNA expression was highest in the swim bladder and gonad, followed by the whole brain. All other tissues tested (esophagus, stomach, anterior intestine, posterior intestine, rectum, liver, kidney, heart, muscle and gill) had mRNA expression levels that were significantly less than whole brain. Toadfish 5-HT2A receptor mRNA expression within the brain was highest in the hindbrain, telencephalon and midbrain/diencephalon regions. Treatment with the 5-HT2A receptor antagonist, ketanserin, resulted in a significant decrease in the pulsatile component of spontaneous urea excretion due to a reduction in urea pulse size with no significant change in pulse frequency. These results lend further support for the 5-HT2A receptor in the regulation of pulsatile urea excretion in toadfish.

AB - Based on early pharmacological work, the serotonin 2A (5-HT2A) receptor subtype is believed to be involved in the regulation of toadfish pulsatile urea excretion. The goal of the following study was to characterize the toadfish 5-HT2A receptor at a molecular level, to determine the tissues in which this receptor is predominantly expressed and to further investigate the pharmacological specificity of toadfish pulsatile urea excretion by examining the effect of ketanserin, a 5-HT2A receptor antagonist, on resting rates of pulsatile urea excretion. The full-length toadfish 5-HT2A receptor encodes a 496 amino acid sequence and shares 57-80% sequence identity to 5-HT2A receptors of other organisms, with 100% conservation among important ligand-binding residues. Toadfish 5-HT2A receptor mRNA expression was highest in the swim bladder and gonad, followed by the whole brain. All other tissues tested (esophagus, stomach, anterior intestine, posterior intestine, rectum, liver, kidney, heart, muscle and gill) had mRNA expression levels that were significantly less than whole brain. Toadfish 5-HT2A receptor mRNA expression within the brain was highest in the hindbrain, telencephalon and midbrain/diencephalon regions. Treatment with the 5-HT2A receptor antagonist, ketanserin, resulted in a significant decrease in the pulsatile component of spontaneous urea excretion due to a reduction in urea pulse size with no significant change in pulse frequency. These results lend further support for the 5-HT2A receptor in the regulation of pulsatile urea excretion in toadfish.

KW - Ammonia

KW - Gill

KW - Gulf toadfish

KW - Nitrogen excretion

KW - Opsanus beta

KW - Swim bladder

KW - TUT

KW - Urea transport

UR - http://www.scopus.com/inward/record.url?scp=84866412186&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866412186&partnerID=8YFLogxK

U2 - 10.1016/j.cbpa.2012.07.013

DO - 10.1016/j.cbpa.2012.07.013

M3 - Article

VL - 163

SP - 319

EP - 326

JO - Comparative Biochemistry & Physiology; A: Comparative Physiology

JF - Comparative Biochemistry & Physiology; A: Comparative Physiology

SN - 1095-6433

IS - 3-4

ER -