The thyroid hormone-inactivating type III deiodinase is expressed in mouse and human β-cells and its targeted inactivation impairs insulin secretion

Mayrin C. Medina, Judith Molina, Yelena Gadea, Alberto Fachado, Monika Murillo, Gordana Simovic, Antonello Pileggi, Arturo Hernández, Helena Edlund, Antonio C. Bianco

Research output: Contribution to journalArticle

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Abstract

Deiodinases are selenoproteins that activate or inactivate thyroid hormone. During vertebrate development, these pathways control thyroid hormone action in a cell-specific fashion explaining how systemic thyroid hormone can affect local control of tissue embryogenesis. Here we investigated the role of the thyroid hormone-inactivating deiodinase (D3) in pancreatic islet function and glucose homeostasis. D3 expression was determined by real-time PCR, immunofluorescence, and enzyme activity. Embryonic and adult wild-type mice and Mice with targeted disruption of Dio3 gene (D3KO) as well as human fetal pancreas and adult islets were studied. Insulin secretion was evaluated in adult mouse isolated islets. We found Dio3 gene expression and protein highly expressed in embryonic and adult pancreatic islets, predominantly in β-cells in both humans and mice. However, mRNA levels were barely detectable for both the thyroid hormone-activating deiodinases types 1 and 2. D3KO animals were found to be glucose intolerant due to in vitro and in vivo impaired glucose-stimulated insulin secretion, without changes in peripheral sensitivity to insulin. D3KO neonatal (postnatal day 0) and adult pancreas exhibited reduced total islet area due to reduced β-cell mass, insulin content, and impaired expression of key β-cells genes. D3 expression in perinatal pancreatic β-cells prevents untimely exposure to thyroid hormone, the absence of which leads to impaired β-cell function and subsequently insulin secretion and glucose homeostasis. An analogous role is likely in humans, given the similar D3 expression pattern.

Original languageEnglish
Pages (from-to)3717-3727
Number of pages11
JournalEndocrinology
Volume152
Issue number10
DOIs
StatePublished - Oct 1 2011
Externally publishedYes

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Thyroid Hormones
Insulin
Glucose
Iodide Peroxidase
Islets of Langerhans
Pancreas
Homeostasis
Selenoproteins
Genes
Embryonic Development
Fluorescent Antibody Technique
Insulin Resistance
Vertebrates
iodothyronine deiodinase type III
Real-Time Polymerase Chain Reaction
Gene Expression
Messenger RNA
Enzymes
Proteins

ASJC Scopus subject areas

  • Endocrinology

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The thyroid hormone-inactivating type III deiodinase is expressed in mouse and human β-cells and its targeted inactivation impairs insulin secretion. / Medina, Mayrin C.; Molina, Judith; Gadea, Yelena; Fachado, Alberto; Murillo, Monika; Simovic, Gordana; Pileggi, Antonello; Hernández, Arturo; Edlund, Helena; Bianco, Antonio C.

In: Endocrinology, Vol. 152, No. 10, 01.10.2011, p. 3717-3727.

Research output: Contribution to journalArticle

Medina, MC, Molina, J, Gadea, Y, Fachado, A, Murillo, M, Simovic, G, Pileggi, A, Hernández, A, Edlund, H & Bianco, AC 2011, 'The thyroid hormone-inactivating type III deiodinase is expressed in mouse and human β-cells and its targeted inactivation impairs insulin secretion', Endocrinology, vol. 152, no. 10, pp. 3717-3727. https://doi.org/10.1210/en.2011-1210
Medina, Mayrin C. ; Molina, Judith ; Gadea, Yelena ; Fachado, Alberto ; Murillo, Monika ; Simovic, Gordana ; Pileggi, Antonello ; Hernández, Arturo ; Edlund, Helena ; Bianco, Antonio C. / The thyroid hormone-inactivating type III deiodinase is expressed in mouse and human β-cells and its targeted inactivation impairs insulin secretion. In: Endocrinology. 2011 ; Vol. 152, No. 10. pp. 3717-3727.
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