The thyroid hormone-inactivating deiodinase functions as a homodimer

G. D.Vivek Sagar, Balázs Gereben, Isabelle Callebaut, Jean Paul Mornon, Anikó Zeöld, Cyntia Curcio-Morelli, John W. Harney, Cristina Luongo, Michelle A. Mulcahey, P. Reed Larsen, Stephen A. Huang, Antonio C. Bianco

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

The type 3 deiodinase (D3) inactivates thyroid hormone action by catalyzing tissue-specific inner ring deiodination, predominantly during embryonic development. D3 has gained much attention as a player in the euthyroid sick syndrome, given its robust reactivation during injury and/or illness. Whereas much of the structure biology of the deiodinases is derived from studies with D2, a dimeric endoplasmic reticulum obligatory activating deiodinase, little is known about the holostructure of the plasma membrane resident D3, the deiodinase capable of thyroid hormone inactivation. Here we used fluorescence resonance energy transfer in live cells to demonstrate that D3 exists as homodimer. While D3 homodimerized in its native state, minor heterodimerization was also observed between D3:D1 and D3:D2 in intact cells, the significance of which remains elusive. Incubation with 0.5-1.2 M urea resulted in loss of D3 homodimerization as assessed by bioluminescence resonance energy transfer and a proportional loss of enzyme activity, to a maximum of approximately 50%. Protein modeling using a D2-based scaffold identified potential dimerization surfaces in the transmembrane and globular domains. Truncation of the transmembrane domain (ΔD3) abrogated dimerization and deiodinase activity except when coexpressed with full-length catalytically inactive deiodinase, thus assembled as ΔD3:D3 dimer; thus the D3 globular domain also exhibits dimerization surfaces. In conclusion, the inactivating deiodinase D3 exists as homo- or heterodimer in living intact cells, a feature that is critical for their catalytic activities.

Original languageEnglish (US)
Pages (from-to)1382-1393
Number of pages12
JournalMolecular Endocrinology
Volume22
Issue number6
DOIs
StatePublished - Jun 2008

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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    Sagar, G. D. V., Gereben, B., Callebaut, I., Mornon, J. P., Zeöld, A., Curcio-Morelli, C., Harney, J. W., Luongo, C., Mulcahey, M. A., Larsen, P. R., Huang, S. A., & Bianco, A. C. (2008). The thyroid hormone-inactivating deiodinase functions as a homodimer. Molecular Endocrinology, 22(6), 1382-1393. https://doi.org/10.1210/me.2007-0490