The TAR-RNA binding protein is required for immunoresponses triggered by Cardiovirus infection

Akihiko Komuro, Yuya Homma, Takaharu Negoro, Glen N. Barber, Curt M. Horvath

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

LGP2 and MDA5 cooperate to detect viral RNA in the cytoplasm of Picornavirus-infected cells and activate innate immune responses. To further define regulatory components of RNA recognition by LGP2/MDA5, a yeast two-hybrid screen was used to identify LGP2-interacting proteins. The screening has identified the TAR-RNA binding protein (TRBP), which is known to be an essential factor for RNA interference (RNAi). Immuno-precipitation experiments demonstrated that TRBP interacted specifically with LGP2 but not with related RIG-I-like receptors, RIG-I or MDA5. siRNA knockdown experiments indicate that TRBP is important for Cardiovirus-triggered interferon responses, but TRBP is not involved in Sendai virus-triggered interferon response that is mediated mainly by RIG-I. To support functional interaction with LGP2, overexpressed TRBP increased Cardiovirus-triggered interferon promoter activity only when LGP2 and MDA5 are co-expressed but not MDA5 alone. Together, our findings illustrate a possible connection between an RNAi-regulatory factor and antiviral RNA recognition that is specifically required for a branch of the virus induced innate immune response.

Original languageEnglish (US)
Pages (from-to)187-193
Number of pages7
JournalBiochemical and biophysical research communications
Volume480
Issue number2
DOIs
StatePublished - Nov 11 2016

Keywords

  • LGP2
  • MDA5
  • Picornavirus
  • RIG-I-like receptors
  • TRBP
  • Type-I interferon

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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