The t(14;18) defines a unique subset of diffuse large B-cell lymphoma with a germinal center B-cell gene expression profile

James Z. Huang, Warren G. Sanger, Timothy C. Greiner, Louis M. Staudt, Dennis D. Weisenburger, Diane L. Pickering, James C. Lynch, James O. Armitage, Roger A. Warnke, Ash A. Alizadeh, Izidore Lossos, Ronald Levy, Wing C. Chan

Research output: Contribution to journalArticle

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Abstract

Recently we have identified subgroups of de novo primary diffuse large B-cell lymphoma (DLBCL) based on complementary DNA microarray-generated gene expression profiles. To correlate the gene expression profiles with cytogenetic abnormalities in these DLBCLs, we examined the occurrence of the t(14;18)(q32; q21) in the 2 distinctive subgroups of DLBCL: one with the germinal center B-cell gene expression signature and the other with the activated B cell-like gene expression signature. The t(14;18) was detected in 7 of 35 cases (20%). All 7 t(14;18)-positive cases had a germinal center B-cell gene expression profile, representing 35% of the cases in this sub-group, and 6 of these 7 cases had very similar gene expression profiles. The expression of bcl-2 and bcl-6 proteins was not significantly different between the t(14;18)-positive and -negative cases, whereas CD10 was detected only in the group with the germinal center B-cell expression profile, and CD10 was most frequently expressed in the t(14;18)-positive cases. This study supports the validity of subdividing DLBCL into 2 major subgroups by gene expression profiling, with the t(14;18) being an important event in the pathogenesis of a subset of DLBCL arising from germinal center B cells. CD10 protein expression is useful in identifying cases of DLBCL with a germinal center B-cell gene expression profile and is often expressed in cases with the t(14;18).

Original languageEnglish
Pages (from-to)2285-2290
Number of pages6
JournalBlood
Volume99
Issue number7
DOIs
StatePublished - Apr 1 2002
Externally publishedYes

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Germinal Center
Lymphoma, Large B-Cell, Diffuse
Transcriptome
Gene expression
B-Lymphocytes
Cells
Gene Expression Profiling
Oligonucleotide Array Sequence Analysis
Chromosome Aberrations
Microarrays
Proteins
Complementary DNA

ASJC Scopus subject areas

  • Hematology

Cite this

Huang, J. Z., Sanger, W. G., Greiner, T. C., Staudt, L. M., Weisenburger, D. D., Pickering, D. L., ... Chan, W. C. (2002). The t(14;18) defines a unique subset of diffuse large B-cell lymphoma with a germinal center B-cell gene expression profile. Blood, 99(7), 2285-2290. https://doi.org/10.1182/blood.V99.7.2285

The t(14;18) defines a unique subset of diffuse large B-cell lymphoma with a germinal center B-cell gene expression profile. / Huang, James Z.; Sanger, Warren G.; Greiner, Timothy C.; Staudt, Louis M.; Weisenburger, Dennis D.; Pickering, Diane L.; Lynch, James C.; Armitage, James O.; Warnke, Roger A.; Alizadeh, Ash A.; Lossos, Izidore; Levy, Ronald; Chan, Wing C.

In: Blood, Vol. 99, No. 7, 01.04.2002, p. 2285-2290.

Research output: Contribution to journalArticle

Huang, JZ, Sanger, WG, Greiner, TC, Staudt, LM, Weisenburger, DD, Pickering, DL, Lynch, JC, Armitage, JO, Warnke, RA, Alizadeh, AA, Lossos, I, Levy, R & Chan, WC 2002, 'The t(14;18) defines a unique subset of diffuse large B-cell lymphoma with a germinal center B-cell gene expression profile', Blood, vol. 99, no. 7, pp. 2285-2290. https://doi.org/10.1182/blood.V99.7.2285
Huang JZ, Sanger WG, Greiner TC, Staudt LM, Weisenburger DD, Pickering DL et al. The t(14;18) defines a unique subset of diffuse large B-cell lymphoma with a germinal center B-cell gene expression profile. Blood. 2002 Apr 1;99(7):2285-2290. https://doi.org/10.1182/blood.V99.7.2285
Huang, James Z. ; Sanger, Warren G. ; Greiner, Timothy C. ; Staudt, Louis M. ; Weisenburger, Dennis D. ; Pickering, Diane L. ; Lynch, James C. ; Armitage, James O. ; Warnke, Roger A. ; Alizadeh, Ash A. ; Lossos, Izidore ; Levy, Ronald ; Chan, Wing C. / The t(14;18) defines a unique subset of diffuse large B-cell lymphoma with a germinal center B-cell gene expression profile. In: Blood. 2002 ; Vol. 99, No. 7. pp. 2285-2290.
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AU - Staudt, Louis M.

AU - Weisenburger, Dennis D.

AU - Pickering, Diane L.

AU - Lynch, James C.

AU - Armitage, James O.

AU - Warnke, Roger A.

AU - Alizadeh, Ash A.

AU - Lossos, Izidore

AU - Levy, Ronald

AU - Chan, Wing C.

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N2 - Recently we have identified subgroups of de novo primary diffuse large B-cell lymphoma (DLBCL) based on complementary DNA microarray-generated gene expression profiles. To correlate the gene expression profiles with cytogenetic abnormalities in these DLBCLs, we examined the occurrence of the t(14;18)(q32; q21) in the 2 distinctive subgroups of DLBCL: one with the germinal center B-cell gene expression signature and the other with the activated B cell-like gene expression signature. The t(14;18) was detected in 7 of 35 cases (20%). All 7 t(14;18)-positive cases had a germinal center B-cell gene expression profile, representing 35% of the cases in this sub-group, and 6 of these 7 cases had very similar gene expression profiles. The expression of bcl-2 and bcl-6 proteins was not significantly different between the t(14;18)-positive and -negative cases, whereas CD10 was detected only in the group with the germinal center B-cell expression profile, and CD10 was most frequently expressed in the t(14;18)-positive cases. This study supports the validity of subdividing DLBCL into 2 major subgroups by gene expression profiling, with the t(14;18) being an important event in the pathogenesis of a subset of DLBCL arising from germinal center B cells. CD10 protein expression is useful in identifying cases of DLBCL with a germinal center B-cell gene expression profile and is often expressed in cases with the t(14;18).

AB - Recently we have identified subgroups of de novo primary diffuse large B-cell lymphoma (DLBCL) based on complementary DNA microarray-generated gene expression profiles. To correlate the gene expression profiles with cytogenetic abnormalities in these DLBCLs, we examined the occurrence of the t(14;18)(q32; q21) in the 2 distinctive subgroups of DLBCL: one with the germinal center B-cell gene expression signature and the other with the activated B cell-like gene expression signature. The t(14;18) was detected in 7 of 35 cases (20%). All 7 t(14;18)-positive cases had a germinal center B-cell gene expression profile, representing 35% of the cases in this sub-group, and 6 of these 7 cases had very similar gene expression profiles. The expression of bcl-2 and bcl-6 proteins was not significantly different between the t(14;18)-positive and -negative cases, whereas CD10 was detected only in the group with the germinal center B-cell expression profile, and CD10 was most frequently expressed in the t(14;18)-positive cases. This study supports the validity of subdividing DLBCL into 2 major subgroups by gene expression profiling, with the t(14;18) being an important event in the pathogenesis of a subset of DLBCL arising from germinal center B cells. CD10 protein expression is useful in identifying cases of DLBCL with a germinal center B-cell gene expression profile and is often expressed in cases with the t(14;18).

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