The stromal composition of malignant lymphoid aggregates in bone marrow: Variations in architecture and phenotype in different B-cell tumours

Francisco Vega, L. Jeffrey Medeiros, Wen Hua Lang, Adnan Mansoor, Carlos Bueso-Ramos, Dan Jones

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

We present evidence that different B-cell tumours, in bone marrow, have different relationships to stroma. Marrow core biopsies from 46 patients with B-cell tumours were immunostained with antibodies for distinct stromal cells. Cases included follicular lymphoma (FL), chronic lymphocytic leukaemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma (MCL), lympho-plasmacytic lymphoma (LPL), and nodal, extranodal and splenic marginal zone lymphoma (NMZL, MALT, SMZL). In normal marrow, low-affinity nerve growth factor receptor (LNGFR) highlighted a fine network of adventitial reticular cells (ARC). The nodular aggregates of CLL/SLL, NMZL, MALT and SMZL were characterized by distortion of the ARC network and downregulation of LNGFR. In contrast, the aggregates of FL, LPL and MCL were composed of linear arrays of ARC in tight association with individual tumour cells. LNFGR+ was upregulated in ARC associated with the aggregates in FL, LPL and focally in MCL. Upregulation of CD35, vascular cell adhesion molecule (VCAM-1) and CD40 on ARC was noted exclusively in FL. Marrow lymphoid aggregates in CLL/SLL, NMZL, MALT and SMZL probably grow by displacing the pre-existing marrow stroma, while FL and LPL maintain a close association with the ARC network. In FL, expression of follicular dendritic cell-associated markers is modulated in pre-existing marrow stromal cells.

Original languageEnglish
Pages (from-to)569-576
Number of pages8
JournalBritish Journal of Haematology
Volume117
Issue number3
DOIs
StatePublished - Jun 1 2002
Externally publishedYes

Fingerprint

Adventitia
Follicular Lymphoma
B-Cell Chronic Lymphocytic Leukemia
B-Lymphocytes
Bone Marrow
Phenotype
Mantle-Cell Lymphoma
Lymphoma
Neoplasms
Nerve Growth Factor Receptor
Vascular Cell Adhesion Molecule-1
Stromal Cells
Follicular Dendritic Cells
Marginal Zone B-Cell Lymphoma
Up-Regulation
Down-Regulation
Biopsy
Antibodies

Keywords

  • Adventitial/perisinusoidal reticular cells
  • B-cell lymphoma
  • Bone marrow stroma
  • Immunohistochemistry
  • Low-affinity nerve growth factor

ASJC Scopus subject areas

  • Hematology

Cite this

The stromal composition of malignant lymphoid aggregates in bone marrow : Variations in architecture and phenotype in different B-cell tumours. / Vega, Francisco; Medeiros, L. Jeffrey; Lang, Wen Hua; Mansoor, Adnan; Bueso-Ramos, Carlos; Jones, Dan.

In: British Journal of Haematology, Vol. 117, No. 3, 01.06.2002, p. 569-576.

Research output: Contribution to journalArticle

Vega, Francisco ; Medeiros, L. Jeffrey ; Lang, Wen Hua ; Mansoor, Adnan ; Bueso-Ramos, Carlos ; Jones, Dan. / The stromal composition of malignant lymphoid aggregates in bone marrow : Variations in architecture and phenotype in different B-cell tumours. In: British Journal of Haematology. 2002 ; Vol. 117, No. 3. pp. 569-576.
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