The simultaneous determination of both the quantity and the fatty acid composition of the triglycerides in three to ten microliters of plasma

Cyril Y. Bowers; James G. Hamilton; James E. Muldrey; Walter T. Miyamasu; George Ann Reynolds; Andrew V. Schally

doi:10.1007/BF02637267

The simultaneous determination of both the quantity and the fatty acid composition of the triglycerides in three to ten microliters of plasma

Cyril Y. Bowers, James G. Hamilton, James E. Muldrey, Walter T. Miyamasu, George Ann Reynolds, Andrew V. Schally

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

An integrated system using chromatography on silica gel-impregnated glass paper, an internal standard and gas-liquid chromatography (GLC) makes possible the isolation of triglycerides, the determination of triglyceride, fatty acid composition, and the quantitative determination of triglyceride. Recoveries are good and values for total triglyceride obtained by this system compare favorably with values obtained by application of the hydroxamate colorimetric method to the triglyceride eluate from silicic acid columns. The fatty acid composition of the cholesteryl ester (CE) fraction is obtainable at the same time by GLC of methyl esters prepared from the CE fractions which also appears as a distinct spot on these chromatograms. Also, a densitometric method is described for triglyceride determination by photometry of the charred spots obtained after chromatography on the gel-paper.

Original language	English (US)
Pages (from-to)	2-6
Number of pages	5
Journal	Journal of the American Oil Chemists Society
Volume	43
Issue number	1
DOIs	https://doi.org/10.1007/BF02637267
State	Published - Jan 1966
Externally published	Yes

ASJC Scopus subject areas

Chemical Engineering(all)
Organic Chemistry

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10.1007/BF02637267

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The simultaneous determination of both the quantity and the fatty acid composition of the triglycerides in three to ten microliters of plasma. / Bowers, Cyril Y.; Hamilton, James G.; Muldrey, James E.; Miyamasu, Walter T.; Reynolds, George Ann; Schally, Andrew V.

In: Journal of the American Oil Chemists Society, Vol. 43, No. 1, 01.1966, p. 2-6.

Research output: Contribution to journal › Article › peer-review

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abstract = "An integrated system using chromatography on silica gel-impregnated glass paper, an internal standard and gas-liquid chromatography (GLC) makes possible the isolation of triglycerides, the determination of triglyceride, fatty acid composition, and the quantitative determination of triglyceride. Recoveries are good and values for total triglyceride obtained by this system compare favorably with values obtained by application of the hydroxamate colorimetric method to the triglyceride eluate from silicic acid columns. The fatty acid composition of the cholesteryl ester (CE) fraction is obtainable at the same time by GLC of methyl esters prepared from the CE fractions which also appears as a distinct spot on these chromatograms. Also, a densitometric method is described for triglyceride determination by photometry of the charred spots obtained after chromatography on the gel-paper.",

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