The simian immunodeficiency virus envelope glycoprotein contains two epitopes presented by the Mamu-A*01 class I molecule

Michelle Furchner, Ann L. Erickson, Todd Allen, David Watkins, Alessandro Sette, Philip R. Johnson, Christopher M. Walker

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Cytotoxic T lymphocyte (CTL) responses against the simian immunodeficiency virus (SIV) envelope and Gag proteins were monitored in a Mamu-A*01-positive rhesus macaque infected with SIVsmE660. Peripheral blood mononuclear cells (PBMC) cultured with synthetic peptides spanning the entire gp160 and Gag coding region recognized a total of three epitopes. One located in Gag was identified as the previously described Mamu-A*01-restricted p11cC→M epitope (CTPYDINQM). The other two epitopes, designated p15m and p54m, were located in the gp160 envelope protein. Both were nine amino acids in length and were predicted to bind Mamu-A*01 because they contained proline and leucine residues at positions 3 and 9, respectively. Indeed, expression of this class I major histocompatibility complex molecule was required for target cell recognition by envelope-specific CD8+ T cells directed against both epitopes. These Mamu-A*01-restricted epitopes in the SIV envelope will be useful for monitoring immune responses in vaccinated or infected animals.

Original languageEnglish
Pages (from-to)8035-8039
Number of pages5
JournalJournal of Virology
Volume73
Issue number10
StatePublished - Sep 29 1999
Externally publishedYes

Fingerprint

Simian immunodeficiency virus
Simian Immunodeficiency Virus
epitopes
glycoproteins
Epitopes
Glycoproteins
Viral Envelope Proteins
gag Gene Products
Immunologic Monitoring
cytotoxic T-lymphocytes
synthetic peptides
major histocompatibility complex
Cytotoxic T-Lymphocytes
mononuclear leukocytes
Macaca mulatta
Major Histocompatibility Complex
Proline
Leucine
leucine
proline

ASJC Scopus subject areas

  • Immunology

Cite this

Furchner, M., Erickson, A. L., Allen, T., Watkins, D., Sette, A., Johnson, P. R., & Walker, C. M. (1999). The simian immunodeficiency virus envelope glycoprotein contains two epitopes presented by the Mamu-A*01 class I molecule. Journal of Virology, 73(10), 8035-8039.

The simian immunodeficiency virus envelope glycoprotein contains two epitopes presented by the Mamu-A*01 class I molecule. / Furchner, Michelle; Erickson, Ann L.; Allen, Todd; Watkins, David; Sette, Alessandro; Johnson, Philip R.; Walker, Christopher M.

In: Journal of Virology, Vol. 73, No. 10, 29.09.1999, p. 8035-8039.

Research output: Contribution to journalArticle

Furchner, M, Erickson, AL, Allen, T, Watkins, D, Sette, A, Johnson, PR & Walker, CM 1999, 'The simian immunodeficiency virus envelope glycoprotein contains two epitopes presented by the Mamu-A*01 class I molecule', Journal of Virology, vol. 73, no. 10, pp. 8035-8039.
Furchner, Michelle ; Erickson, Ann L. ; Allen, Todd ; Watkins, David ; Sette, Alessandro ; Johnson, Philip R. ; Walker, Christopher M. / The simian immunodeficiency virus envelope glycoprotein contains two epitopes presented by the Mamu-A*01 class I molecule. In: Journal of Virology. 1999 ; Vol. 73, No. 10. pp. 8035-8039.
@article{f585c2f0804f456397e7d97ab9b9c6c3,
title = "The simian immunodeficiency virus envelope glycoprotein contains two epitopes presented by the Mamu-A*01 class I molecule",
abstract = "Cytotoxic T lymphocyte (CTL) responses against the simian immunodeficiency virus (SIV) envelope and Gag proteins were monitored in a Mamu-A*01-positive rhesus macaque infected with SIVsmE660. Peripheral blood mononuclear cells (PBMC) cultured with synthetic peptides spanning the entire gp160 and Gag coding region recognized a total of three epitopes. One located in Gag was identified as the previously described Mamu-A*01-restricted p11cC→M epitope (CTPYDINQM). The other two epitopes, designated p15m and p54m, were located in the gp160 envelope protein. Both were nine amino acids in length and were predicted to bind Mamu-A*01 because they contained proline and leucine residues at positions 3 and 9, respectively. Indeed, expression of this class I major histocompatibility complex molecule was required for target cell recognition by envelope-specific CD8+ T cells directed against both epitopes. These Mamu-A*01-restricted epitopes in the SIV envelope will be useful for monitoring immune responses in vaccinated or infected animals.",
author = "Michelle Furchner and Erickson, {Ann L.} and Todd Allen and David Watkins and Alessandro Sette and Johnson, {Philip R.} and Walker, {Christopher M.}",
year = "1999",
month = "9",
day = "29",
language = "English",
volume = "73",
pages = "8035--8039",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "10",

}

TY - JOUR

T1 - The simian immunodeficiency virus envelope glycoprotein contains two epitopes presented by the Mamu-A*01 class I molecule

AU - Furchner, Michelle

AU - Erickson, Ann L.

AU - Allen, Todd

AU - Watkins, David

AU - Sette, Alessandro

AU - Johnson, Philip R.

AU - Walker, Christopher M.

PY - 1999/9/29

Y1 - 1999/9/29

N2 - Cytotoxic T lymphocyte (CTL) responses against the simian immunodeficiency virus (SIV) envelope and Gag proteins were monitored in a Mamu-A*01-positive rhesus macaque infected with SIVsmE660. Peripheral blood mononuclear cells (PBMC) cultured with synthetic peptides spanning the entire gp160 and Gag coding region recognized a total of three epitopes. One located in Gag was identified as the previously described Mamu-A*01-restricted p11cC→M epitope (CTPYDINQM). The other two epitopes, designated p15m and p54m, were located in the gp160 envelope protein. Both were nine amino acids in length and were predicted to bind Mamu-A*01 because they contained proline and leucine residues at positions 3 and 9, respectively. Indeed, expression of this class I major histocompatibility complex molecule was required for target cell recognition by envelope-specific CD8+ T cells directed against both epitopes. These Mamu-A*01-restricted epitopes in the SIV envelope will be useful for monitoring immune responses in vaccinated or infected animals.

AB - Cytotoxic T lymphocyte (CTL) responses against the simian immunodeficiency virus (SIV) envelope and Gag proteins were monitored in a Mamu-A*01-positive rhesus macaque infected with SIVsmE660. Peripheral blood mononuclear cells (PBMC) cultured with synthetic peptides spanning the entire gp160 and Gag coding region recognized a total of three epitopes. One located in Gag was identified as the previously described Mamu-A*01-restricted p11cC→M epitope (CTPYDINQM). The other two epitopes, designated p15m and p54m, were located in the gp160 envelope protein. Both were nine amino acids in length and were predicted to bind Mamu-A*01 because they contained proline and leucine residues at positions 3 and 9, respectively. Indeed, expression of this class I major histocompatibility complex molecule was required for target cell recognition by envelope-specific CD8+ T cells directed against both epitopes. These Mamu-A*01-restricted epitopes in the SIV envelope will be useful for monitoring immune responses in vaccinated or infected animals.

UR - http://www.scopus.com/inward/record.url?scp=0032823285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032823285&partnerID=8YFLogxK

M3 - Article

C2 - 10482552

AN - SCOPUS:0032823285

VL - 73

SP - 8035

EP - 8039

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 10

ER -