The simian immunodeficiency virus envelope glycoprotein contains two epitopes presented by the Mamu-A*01 class I molecule

Michelle Furchner, Ann L. Erickson, Todd Allen, David I. Watkins, Alessandro Sette, Philip R. Johnson, Christopher M. Walker

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Cytotoxic T lymphocyte (CTL) responses against the simian immunodeficiency virus (SIV) envelope and Gag proteins were monitored in a Mamu-A*01-positive rhesus macaque infected with SIVsmE660. Peripheral blood mononuclear cells (PBMC) cultured with synthetic peptides spanning the entire gp160 and Gag coding region recognized a total of three epitopes. One located in Gag was identified as the previously described Mamu-A*01-restricted p11cC→M epitope (CTPYDINQM). The other two epitopes, designated p15m and p54m, were located in the gp160 envelope protein. Both were nine amino acids in length and were predicted to bind Mamu-A*01 because they contained proline and leucine residues at positions 3 and 9, respectively. Indeed, expression of this class I major histocompatibility complex molecule was required for target cell recognition by envelope-specific CD8+ T cells directed against both epitopes. These Mamu-A*01-restricted epitopes in the SIV envelope will be useful for monitoring immune responses in vaccinated or infected animals.

Original languageEnglish (US)
Pages (from-to)8035-8039
Number of pages5
JournalJournal of virology
Volume73
Issue number10
StatePublished - Sep 29 1999
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Furchner, M., Erickson, A. L., Allen, T., Watkins, D. I., Sette, A., Johnson, P. R., & Walker, C. M. (1999). The simian immunodeficiency virus envelope glycoprotein contains two epitopes presented by the Mamu-A*01 class I molecule. Journal of virology, 73(10), 8035-8039.