Abstract
Cytotoxic T lymphocyte (CTL) responses against the simian immunodeficiency virus (SIV) envelope and Gag proteins were monitored in a Mamu-A*01-positive rhesus macaque infected with SIVsmE660. Peripheral blood mononuclear cells (PBMC) cultured with synthetic peptides spanning the entire gp160 and Gag coding region recognized a total of three epitopes. One located in Gag was identified as the previously described Mamu-A*01-restricted p11cC→M epitope (CTPYDINQM). The other two epitopes, designated p15m and p54m, were located in the gp160 envelope protein. Both were nine amino acids in length and were predicted to bind Mamu-A*01 because they contained proline and leucine residues at positions 3 and 9, respectively. Indeed, expression of this class I major histocompatibility complex molecule was required for target cell recognition by envelope-specific CD8+ T cells directed against both epitopes. These Mamu-A*01-restricted epitopes in the SIV envelope will be useful for monitoring immune responses in vaccinated or infected animals.
| Original language | English |
|---|---|
| Pages (from-to) | 8035-8039 |
| Number of pages | 5 |
| Journal | Journal of Virology |
| Volume | 73 |
| Issue number | 10 |
| State | Published - Sep 29 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
The simian immunodeficiency virus envelope glycoprotein contains two epitopes presented by the Mamu-A*01 class I molecule. / Furchner, Michelle; Erickson, Ann L.; Allen, Todd; Watkins, David; Sette, Alessandro; Johnson, Philip R.; Walker, Christopher M.
In: Journal of Virology, Vol. 73, No. 10, 29.09.1999, p. 8035-8039.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The simian immunodeficiency virus envelope glycoprotein contains two epitopes presented by the Mamu-A*01 class I molecule
AU - Furchner, Michelle
AU - Erickson, Ann L.
AU - Allen, Todd
AU - Watkins, David
AU - Sette, Alessandro
AU - Johnson, Philip R.
AU - Walker, Christopher M.
PY - 1999/9/29
Y1 - 1999/9/29
N2 - Cytotoxic T lymphocyte (CTL) responses against the simian immunodeficiency virus (SIV) envelope and Gag proteins were monitored in a Mamu-A*01-positive rhesus macaque infected with SIVsmE660. Peripheral blood mononuclear cells (PBMC) cultured with synthetic peptides spanning the entire gp160 and Gag coding region recognized a total of three epitopes. One located in Gag was identified as the previously described Mamu-A*01-restricted p11cC→M epitope (CTPYDINQM). The other two epitopes, designated p15m and p54m, were located in the gp160 envelope protein. Both were nine amino acids in length and were predicted to bind Mamu-A*01 because they contained proline and leucine residues at positions 3 and 9, respectively. Indeed, expression of this class I major histocompatibility complex molecule was required for target cell recognition by envelope-specific CD8+ T cells directed against both epitopes. These Mamu-A*01-restricted epitopes in the SIV envelope will be useful for monitoring immune responses in vaccinated or infected animals.
AB - Cytotoxic T lymphocyte (CTL) responses against the simian immunodeficiency virus (SIV) envelope and Gag proteins were monitored in a Mamu-A*01-positive rhesus macaque infected with SIVsmE660. Peripheral blood mononuclear cells (PBMC) cultured with synthetic peptides spanning the entire gp160 and Gag coding region recognized a total of three epitopes. One located in Gag was identified as the previously described Mamu-A*01-restricted p11cC→M epitope (CTPYDINQM). The other two epitopes, designated p15m and p54m, were located in the gp160 envelope protein. Both were nine amino acids in length and were predicted to bind Mamu-A*01 because they contained proline and leucine residues at positions 3 and 9, respectively. Indeed, expression of this class I major histocompatibility complex molecule was required for target cell recognition by envelope-specific CD8+ T cells directed against both epitopes. These Mamu-A*01-restricted epitopes in the SIV envelope will be useful for monitoring immune responses in vaccinated or infected animals.
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UR - http://www.scopus.com/inward/citedby.url?scp=0032823285&partnerID=8YFLogxK
M3 - Article
C2 - 10482552
AN - SCOPUS:0032823285
VL - 73
SP - 8035
EP - 8039
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 10
ER -