The significance of nitric oxide production in the brain after injury

Ahmad Khaldi, C. C. Chiueh, M. Ross Bullock, J. J. Woodward

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Glutamate toxicity has been implicated in many aspects of brain injury including traumatic, ischemic, and hemorrhagic damage. We have used in vitro as well as in vivo methods to measure NO production and to examine the role of NO in glutamate toxicity. In building our recombinant system, we used human kidney embryonic cells, HEK 293, as host for transfection of nNOS and NMDA receptor proteins. Cells cotransfected with NMDA and nNOS were more resistant to glutamate toxicity. This resistance correlated with NO production as measured by citrulline assay. Meanwhile, the production of NO did not significantly change the response of the NMDA receptor as seen by calcium studies. Moreover, in vivo, NO production was directly correlated with brain tissue oxygen tension in subarachnoid hemorrhage patients. These data and others point toward the importance of NO production in the response of brain to injury.

Original languageEnglish (US)
Pages (from-to)53-59
Number of pages7
JournalAnnals of the New York Academy of Sciences
Volume962
DOIs
StatePublished - 2002

Keywords

  • Brain injury
  • Nitric oxide production
  • NMDA receptor

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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