Abstract
Three types of sensory neurones and two kinds of motor neurones in the segmental ganglion of the leech were examined with the light and electron microscope after intracellular injection of horseradish peroxidase (HRP) for a histological marker. The aim was to develop a method for identifying the synapses of specific cells in the ganglion's complex neuropil and to form a picture of their distribution and structure. Reaction of HRP with different benzadine derivatives produces opaque and electron dense deposits. For light microscopy a blue stain is formed that makes processes visible in whole mounts millimeters away from the injection site at the soma. The reaction product for electron microscopy is distributed throughout the cytoplasm, yet ultrastructural details are preserved.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 481-499 |
| Number of pages | 19 |
| Journal | Proceedings of the Royal Society of London - Biological Sciences |
| Volume | 194 |
| Issue number | 1117 |
| DOIs | |
| State | Published - Jan 1 1976 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)
- Environmental Science(all)
- Agricultural and Biological Sciences(all)