The shape of the ganglion cell plus inner plexiform layers of the normal human macula

Robert W. Knighton, Giovanni Gregori

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

PURPOSE. To use surfaces generated by two-dimensional penalized splines (2D P-splines) to characterize the shape of the macular ganglion cell plus inner plexiform layers (GCL{thorn}IPL) in a group of normal humans. METHODS. Macular images of the right eyes of 23 normal subjects ranging in age from 18 to 75 years were obtained with spectral-domain optical coherence tomography (SD-OCT). The thickness of GCL{thorn}IPL was determined by manual segmentation, areas with blood vessels were removed, and the resulting maps were fit by smooth surfaces in polar coordinates centered on the fovea. RESULTS. Smooth surfaces based on 2D P-splines could precisely represent GCL{thorn}IPL thickness data, with errors comparable to the axial resolution of the SD-OCT instrument. Metrics were developed for the size, shape, and slope of the edge of the foveal depression and size and shape of the surrounding macular ridge. The slope of the foveal edge was negatively correlated with foveal size (r=-0.60). The size of the macular ridge was positively correlated with foveal size (r=0.75), with a slope near unity (0.90 ± 0.18). The centroids of the foveal edge and macular ridge clustered near the foveal center. The foveal edge and macular ridge were well fit by ellipses. The mean GCL{thorn}IPL thickness formed an elliptical annulus elongated by approximately 30% in the horizontal direction. CONCLUSIONS. The methods developed here provide precise characterization of retinal layers for the study of glaucoma, foveal development, and other applications.

Original languageEnglish (US)
Pages (from-to)7412-7420
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume53
Issue number11
DOIs
StatePublished - Oct 2012

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'The shape of the ganglion cell plus inner plexiform layers of the normal human macula'. Together they form a unique fingerprint.

Cite this