Methods are described for isolating functionally pure C1, C4, C2, C3, C5, C6, C7, C8, C9, C1 inactivator, and C3 inactivator, from a single pool of human serum. Salt precipitation at different pH levels, chromatography on DEAE- or CM-cellulose, and gel filtration through Sephadex were used. The reagents and sheep erythrocyte intermediate complexes used for testing the human components and inactivators, also are described. The yield of components for most columns with cellulose varied from 30 to 50 percent. With Sephadex G-200, 100 per cent of the C9 was recovered. Certain problems in preservation of components and in hemolytic assays using C8 and C9 are outlined briefly.
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