The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides

M. H. Fittkau, P. Zilla, D. Bezuidenhout, M. P. Lutolf, P. Human, J. A. Hubbell, N. Davies

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

The ability of the biomimetic peptides YIGSR, PHSRN and RGD to selectively affect adhesion and migration of human microvascular endothelial cells (MVEC) and vascular smooth muscle cells (HVSMC) was evaluated. Cell mobility was quantified by time-lapse video microscopy of single cells migrating on peptide modified surfaces. Polyethylene glycol (PEG) hydrogels modified with YIGSR or PHSRN allowed only limited adhesion and no spreading of MVEC and HVSMC. However, when these peptides were individually combined with the strong cell binding peptide RGD in PEG hydrogels, the YIGSR peptide was found to selectively enhance the migration of MVEC by 25% over that of MVEC on RGD alone (p<0.05). No corresponding effect was observed for HVSMC. This suggests that the desired response of specific cell types to tissue engineering scaffolds could be optimized through a combinatory approach to the use of biomimetic peptides.

Original languageEnglish
Pages (from-to)167-174
Number of pages8
JournalBiomaterials
Volume26
Issue number2
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Fingerprint

tyrosyl-isoleucyl-glycyl-seryl-arginine
Endothelial cells
Peptides
Endothelial Cells
Modulation
Biomimetics
Hydrogels
prolyl-histidyl-seryl-arginyl-asparagine
Tissue Scaffolds
Video Microscopy
Adhesion
Polyethylene glycols
Tissue Engineering
Vascular Smooth Muscle
Smooth Muscle Myocytes
Tissue engineering
Muscle
Scaffolds (biology)
Microscopic examination
Cells

Keywords

  • Cell mobility
  • Microvascular endothelial cells
  • Peptide
  • Polyethylene glycol
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Fittkau, M. H., Zilla, P., Bezuidenhout, D., Lutolf, M. P., Human, P., Hubbell, J. A., & Davies, N. (2005). The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides. Biomaterials, 26(2), 167-174. https://doi.org/10.1016/j.biomaterials.2004.02.012

The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides. / Fittkau, M. H.; Zilla, P.; Bezuidenhout, D.; Lutolf, M. P.; Human, P.; Hubbell, J. A.; Davies, N.

In: Biomaterials, Vol. 26, No. 2, 01.01.2005, p. 167-174.

Research output: Contribution to journalArticle

Fittkau, MH, Zilla, P, Bezuidenhout, D, Lutolf, MP, Human, P, Hubbell, JA & Davies, N 2005, 'The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides', Biomaterials, vol. 26, no. 2, pp. 167-174. https://doi.org/10.1016/j.biomaterials.2004.02.012
Fittkau, M. H. ; Zilla, P. ; Bezuidenhout, D. ; Lutolf, M. P. ; Human, P. ; Hubbell, J. A. ; Davies, N. / The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides. In: Biomaterials. 2005 ; Vol. 26, No. 2. pp. 167-174.
@article{2647548dbf054759928e994a2391d432,
title = "The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides",
abstract = "The ability of the biomimetic peptides YIGSR, PHSRN and RGD to selectively affect adhesion and migration of human microvascular endothelial cells (MVEC) and vascular smooth muscle cells (HVSMC) was evaluated. Cell mobility was quantified by time-lapse video microscopy of single cells migrating on peptide modified surfaces. Polyethylene glycol (PEG) hydrogels modified with YIGSR or PHSRN allowed only limited adhesion and no spreading of MVEC and HVSMC. However, when these peptides were individually combined with the strong cell binding peptide RGD in PEG hydrogels, the YIGSR peptide was found to selectively enhance the migration of MVEC by 25{\%} over that of MVEC on RGD alone (p<0.05). No corresponding effect was observed for HVSMC. This suggests that the desired response of specific cell types to tissue engineering scaffolds could be optimized through a combinatory approach to the use of biomimetic peptides.",
keywords = "Cell mobility, Microvascular endothelial cells, Peptide, Polyethylene glycol, Tissue engineering",
author = "Fittkau, {M. H.} and P. Zilla and D. Bezuidenhout and Lutolf, {M. P.} and P. Human and Hubbell, {J. A.} and N. Davies",
year = "2005",
month = "1",
day = "1",
doi = "10.1016/j.biomaterials.2004.02.012",
language = "English",
volume = "26",
pages = "167--174",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides

AU - Fittkau, M. H.

AU - Zilla, P.

AU - Bezuidenhout, D.

AU - Lutolf, M. P.

AU - Human, P.

AU - Hubbell, J. A.

AU - Davies, N.

PY - 2005/1/1

Y1 - 2005/1/1

N2 - The ability of the biomimetic peptides YIGSR, PHSRN and RGD to selectively affect adhesion and migration of human microvascular endothelial cells (MVEC) and vascular smooth muscle cells (HVSMC) was evaluated. Cell mobility was quantified by time-lapse video microscopy of single cells migrating on peptide modified surfaces. Polyethylene glycol (PEG) hydrogels modified with YIGSR or PHSRN allowed only limited adhesion and no spreading of MVEC and HVSMC. However, when these peptides were individually combined with the strong cell binding peptide RGD in PEG hydrogels, the YIGSR peptide was found to selectively enhance the migration of MVEC by 25% over that of MVEC on RGD alone (p<0.05). No corresponding effect was observed for HVSMC. This suggests that the desired response of specific cell types to tissue engineering scaffolds could be optimized through a combinatory approach to the use of biomimetic peptides.

AB - The ability of the biomimetic peptides YIGSR, PHSRN and RGD to selectively affect adhesion and migration of human microvascular endothelial cells (MVEC) and vascular smooth muscle cells (HVSMC) was evaluated. Cell mobility was quantified by time-lapse video microscopy of single cells migrating on peptide modified surfaces. Polyethylene glycol (PEG) hydrogels modified with YIGSR or PHSRN allowed only limited adhesion and no spreading of MVEC and HVSMC. However, when these peptides were individually combined with the strong cell binding peptide RGD in PEG hydrogels, the YIGSR peptide was found to selectively enhance the migration of MVEC by 25% over that of MVEC on RGD alone (p<0.05). No corresponding effect was observed for HVSMC. This suggests that the desired response of specific cell types to tissue engineering scaffolds could be optimized through a combinatory approach to the use of biomimetic peptides.

KW - Cell mobility

KW - Microvascular endothelial cells

KW - Peptide

KW - Polyethylene glycol

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=2942741148&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942741148&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2004.02.012

DO - 10.1016/j.biomaterials.2004.02.012

M3 - Article

VL - 26

SP - 167

EP - 174

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 2

ER -