The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides

M. H. Fittkau, P. Zilla, D. Bezuidenhout, M. P. Lutolf, P. Human, J. A. Hubbell, N. Davies

Research output: Contribution to journalArticle

150 Scopus citations

Abstract

The ability of the biomimetic peptides YIGSR, PHSRN and RGD to selectively affect adhesion and migration of human microvascular endothelial cells (MVEC) and vascular smooth muscle cells (HVSMC) was evaluated. Cell mobility was quantified by time-lapse video microscopy of single cells migrating on peptide modified surfaces. Polyethylene glycol (PEG) hydrogels modified with YIGSR or PHSRN allowed only limited adhesion and no spreading of MVEC and HVSMC. However, when these peptides were individually combined with the strong cell binding peptide RGD in PEG hydrogels, the YIGSR peptide was found to selectively enhance the migration of MVEC by 25% over that of MVEC on RGD alone (p<0.05). No corresponding effect was observed for HVSMC. This suggests that the desired response of specific cell types to tissue engineering scaffolds could be optimized through a combinatory approach to the use of biomimetic peptides.

Original languageEnglish (US)
Pages (from-to)167-174
Number of pages8
JournalBiomaterials
Volume26
Issue number2
DOIs
StatePublished - Jan 1 2005

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Keywords

  • Cell mobility
  • Microvascular endothelial cells
  • Peptide
  • Polyethylene glycol
  • Tissue engineering

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Fittkau, M. H., Zilla, P., Bezuidenhout, D., Lutolf, M. P., Human, P., Hubbell, J. A., & Davies, N. (2005). The selective modulation of endothelial cell mobility on RGD peptide containing surfaces by YIGSR peptides. Biomaterials, 26(2), 167-174. https://doi.org/10.1016/j.biomaterials.2004.02.012