The SC5b-7 complex: Formation, isolation, properties, and subunit composition

E. R. Podack, W. P. Kolb, H. J. Muller-Eberhard

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

Activation of C in C8-depleted serum results in the formation of a soluble complex containing C5, C6, and C7. The complex has an electrophoretic mobility of an α-globulin, an s-rate of 18.5S, and a m.w. of 668,000 daltons. This complex was isolated and upon SDS polyacrylamide gel electrophoresis it was found to contain, in addition to C5b, C6 and C7, an 88,000 dalton glycoprotein. The protein was identified as the band V protein of the soluble C5b-9 complex. It is referred to as SIIIs-protein, or S-protein. Since the S-protein does not bind to C5b-6, it is concluded that it is incorporated during the fusion of C5b-6 with C7. The SC5b-7 complex exhibits the same neoantigen as the SC5b-9 complex, but compared to the C5b-6 complex it appears to contain an additionally qualitatively distinct neoantigen.

Original languageEnglish
Pages (from-to)2024-2029
Number of pages6
JournalJournal of Immunology
Volume119
Issue number6
StatePublished - Dec 1 1977
Externally publishedYes

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Protein S
Proteins
Globulins
Polyacrylamide Gel Electrophoresis
Glycoproteins
Serum
complement C5b-7 complex
complement C5b-6 complex
SC5b-9 protein complex

ASJC Scopus subject areas

  • Immunology

Cite this

Podack, E. R., Kolb, W. P., & Muller-Eberhard, H. J. (1977). The SC5b-7 complex: Formation, isolation, properties, and subunit composition. Journal of Immunology, 119(6), 2024-2029.

The SC5b-7 complex : Formation, isolation, properties, and subunit composition. / Podack, E. R.; Kolb, W. P.; Muller-Eberhard, H. J.

In: Journal of Immunology, Vol. 119, No. 6, 01.12.1977, p. 2024-2029.

Research output: Contribution to journalArticle

Podack, ER, Kolb, WP & Muller-Eberhard, HJ 1977, 'The SC5b-7 complex: Formation, isolation, properties, and subunit composition', Journal of Immunology, vol. 119, no. 6, pp. 2024-2029.
Podack ER, Kolb WP, Muller-Eberhard HJ. The SC5b-7 complex: Formation, isolation, properties, and subunit composition. Journal of Immunology. 1977 Dec 1;119(6):2024-2029.
Podack, E. R. ; Kolb, W. P. ; Muller-Eberhard, H. J. / The SC5b-7 complex : Formation, isolation, properties, and subunit composition. In: Journal of Immunology. 1977 ; Vol. 119, No. 6. pp. 2024-2029.
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