The role of type I collagen in aortic wall strength with a homotrimeric [α1(I)]3 collagen mouse model

Angela G. Vouyouka, Brent Pfeiffer, Timothy K. Liem, Timothy A. Taylor, Junaid Mudaliar, Charlotte L. Phillips

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Purpose: Elastin and collagen (types I and III) are the primary load-bearing elements in aortic tissue. Deficiencies and derangements in elastin and type III collagen have been associated with the development of aneurysmal disease. However, the role of type I collagen is less well defined. The purpose of this study was to define the role of type I collagen in maintaining biomechanical integrity in the thoracic aorta, with a mouse model that produces homotrimeric type I collagen [α1(I)]3, rather than the normally present heterotrimeric [α1(I)]2 α2(I) type I collagen isotype. Methods: Ascending and descending thoracic aortas from homozygous (oim/oim), heterozygous (oim/+), and wildtype (+/+) mice were harvested. Circumferential and longitudinal load-extension curves were used as a means of determining maximum breaking strength (Fmax) and incremental elastic modulus (IEM). Histologic analyses and hydroxyproline assays were performed as a means of determining collagen organization and content. Results: Circumferentially, the ascending and descending aortas of oim/oim mice demonstrated significantly reduced Fmax, with an Fmax of only 60% and 23%, respectively, of wildtype mice aortas. Oim/oim descending aortas demonstrated significantly greater compliance (decreased IEM), and the ascending aortas also exhibited a trend toward increased compliance. Reduced breaking strength was also demonstrated with longitudinal extension of the descending aorta. Conclusion: The presence of homotrimeric type I collagen isotype (absence of α2(I) collagen) significantly weakens the aorta. This study demonstrates the integral role of type I collagen in the biomechanical and functional properties of the aorta and may help to elucidate the role of collagen in the deveopment of aneurysmal aortic disease or dissection.

Original languageEnglish
Pages (from-to)1263-1270
Number of pages8
JournalJournal of Vascular Surgery
Volume33
Issue number6
DOIs
StatePublished - Jun 1 2001
Externally publishedYes

Fingerprint

Collagen Type I
Thoracic Aorta
Collagen
Aorta
Collagen Type III
Elastin
Elastic Modulus
Compliance
Aortic Diseases
Hydroxyproline
Weight-Bearing
Dissection

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

The role of type I collagen in aortic wall strength with a homotrimeric [α1(I)]3 collagen mouse model. / Vouyouka, Angela G.; Pfeiffer, Brent; Liem, Timothy K.; Taylor, Timothy A.; Mudaliar, Junaid; Phillips, Charlotte L.

In: Journal of Vascular Surgery, Vol. 33, No. 6, 01.06.2001, p. 1263-1270.

Research output: Contribution to journalArticle

Vouyouka, Angela G. ; Pfeiffer, Brent ; Liem, Timothy K. ; Taylor, Timothy A. ; Mudaliar, Junaid ; Phillips, Charlotte L. / The role of type I collagen in aortic wall strength with a homotrimeric [α1(I)]3 collagen mouse model. In: Journal of Vascular Surgery. 2001 ; Vol. 33, No. 6. pp. 1263-1270.
@article{b0074c3e7ed840f39603654ba9fb4fad,
title = "The role of type I collagen in aortic wall strength with a homotrimeric [α1(I)]3 collagen mouse model",
abstract = "Purpose: Elastin and collagen (types I and III) are the primary load-bearing elements in aortic tissue. Deficiencies and derangements in elastin and type III collagen have been associated with the development of aneurysmal disease. However, the role of type I collagen is less well defined. The purpose of this study was to define the role of type I collagen in maintaining biomechanical integrity in the thoracic aorta, with a mouse model that produces homotrimeric type I collagen [α1(I)]3, rather than the normally present heterotrimeric [α1(I)]2 α2(I) type I collagen isotype. Methods: Ascending and descending thoracic aortas from homozygous (oim/oim), heterozygous (oim/+), and wildtype (+/+) mice were harvested. Circumferential and longitudinal load-extension curves were used as a means of determining maximum breaking strength (Fmax) and incremental elastic modulus (IEM). Histologic analyses and hydroxyproline assays were performed as a means of determining collagen organization and content. Results: Circumferentially, the ascending and descending aortas of oim/oim mice demonstrated significantly reduced Fmax, with an Fmax of only 60{\%} and 23{\%}, respectively, of wildtype mice aortas. Oim/oim descending aortas demonstrated significantly greater compliance (decreased IEM), and the ascending aortas also exhibited a trend toward increased compliance. Reduced breaking strength was also demonstrated with longitudinal extension of the descending aorta. Conclusion: The presence of homotrimeric type I collagen isotype (absence of α2(I) collagen) significantly weakens the aorta. This study demonstrates the integral role of type I collagen in the biomechanical and functional properties of the aorta and may help to elucidate the role of collagen in the deveopment of aneurysmal aortic disease or dissection.",
author = "Vouyouka, {Angela G.} and Brent Pfeiffer and Liem, {Timothy K.} and Taylor, {Timothy A.} and Junaid Mudaliar and Phillips, {Charlotte L.}",
year = "2001",
month = "6",
day = "1",
doi = "10.1067/mva.2001.113579",
language = "English",
volume = "33",
pages = "1263--1270",
journal = "Journal of Vascular Surgery",
issn = "0741-5214",
publisher = "Mosby Inc.",
number = "6",

}

TY - JOUR

T1 - The role of type I collagen in aortic wall strength with a homotrimeric [α1(I)]3 collagen mouse model

AU - Vouyouka, Angela G.

AU - Pfeiffer, Brent

AU - Liem, Timothy K.

AU - Taylor, Timothy A.

AU - Mudaliar, Junaid

AU - Phillips, Charlotte L.

PY - 2001/6/1

Y1 - 2001/6/1

N2 - Purpose: Elastin and collagen (types I and III) are the primary load-bearing elements in aortic tissue. Deficiencies and derangements in elastin and type III collagen have been associated with the development of aneurysmal disease. However, the role of type I collagen is less well defined. The purpose of this study was to define the role of type I collagen in maintaining biomechanical integrity in the thoracic aorta, with a mouse model that produces homotrimeric type I collagen [α1(I)]3, rather than the normally present heterotrimeric [α1(I)]2 α2(I) type I collagen isotype. Methods: Ascending and descending thoracic aortas from homozygous (oim/oim), heterozygous (oim/+), and wildtype (+/+) mice were harvested. Circumferential and longitudinal load-extension curves were used as a means of determining maximum breaking strength (Fmax) and incremental elastic modulus (IEM). Histologic analyses and hydroxyproline assays were performed as a means of determining collagen organization and content. Results: Circumferentially, the ascending and descending aortas of oim/oim mice demonstrated significantly reduced Fmax, with an Fmax of only 60% and 23%, respectively, of wildtype mice aortas. Oim/oim descending aortas demonstrated significantly greater compliance (decreased IEM), and the ascending aortas also exhibited a trend toward increased compliance. Reduced breaking strength was also demonstrated with longitudinal extension of the descending aorta. Conclusion: The presence of homotrimeric type I collagen isotype (absence of α2(I) collagen) significantly weakens the aorta. This study demonstrates the integral role of type I collagen in the biomechanical and functional properties of the aorta and may help to elucidate the role of collagen in the deveopment of aneurysmal aortic disease or dissection.

AB - Purpose: Elastin and collagen (types I and III) are the primary load-bearing elements in aortic tissue. Deficiencies and derangements in elastin and type III collagen have been associated with the development of aneurysmal disease. However, the role of type I collagen is less well defined. The purpose of this study was to define the role of type I collagen in maintaining biomechanical integrity in the thoracic aorta, with a mouse model that produces homotrimeric type I collagen [α1(I)]3, rather than the normally present heterotrimeric [α1(I)]2 α2(I) type I collagen isotype. Methods: Ascending and descending thoracic aortas from homozygous (oim/oim), heterozygous (oim/+), and wildtype (+/+) mice were harvested. Circumferential and longitudinal load-extension curves were used as a means of determining maximum breaking strength (Fmax) and incremental elastic modulus (IEM). Histologic analyses and hydroxyproline assays were performed as a means of determining collagen organization and content. Results: Circumferentially, the ascending and descending aortas of oim/oim mice demonstrated significantly reduced Fmax, with an Fmax of only 60% and 23%, respectively, of wildtype mice aortas. Oim/oim descending aortas demonstrated significantly greater compliance (decreased IEM), and the ascending aortas also exhibited a trend toward increased compliance. Reduced breaking strength was also demonstrated with longitudinal extension of the descending aorta. Conclusion: The presence of homotrimeric type I collagen isotype (absence of α2(I) collagen) significantly weakens the aorta. This study demonstrates the integral role of type I collagen in the biomechanical and functional properties of the aorta and may help to elucidate the role of collagen in the deveopment of aneurysmal aortic disease or dissection.

UR - http://www.scopus.com/inward/record.url?scp=0035379734&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035379734&partnerID=8YFLogxK

U2 - 10.1067/mva.2001.113579

DO - 10.1067/mva.2001.113579

M3 - Article

C2 - 11389427

AN - SCOPUS:0035379734

VL - 33

SP - 1263

EP - 1270

JO - Journal of Vascular Surgery

JF - Journal of Vascular Surgery

SN - 0741-5214

IS - 6

ER -