The role of PNP enzyme in autologous rosette-forming cells

P. Goldschmidt-Clermont, M. Petrini, N. Khansari, H. H. Fudenberg

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Abstract

Since purine nucleoside phosphorylase has been associated with suppressor function in lymphocytes, enzyme activities were studied in autologous rosette-forming cells, a subset showing suppressor properties. Levels of this enzyme were higher in these cells than in other T cells. Con A induction of autologous red cell receptors and suppressor activity of T cells were both inhibited in dose-dependent fashion by Formycin B, a well known inhibitor of purine nucleoside phosphorylase. Inhibition of autologous rosette-forming cells was obtained after pulse treatment of cells with Formycin B for as little as 1 hr, whereas cell proliferation was only inhibited when Formycin B was present throughout culture; this confirms the independence of cell proliferation, and development of red cell receptors and suppressor activity. This study indicates a crucial role for purine nucleoside phosphoylase enzyme in induction of T cell suppressor activity.

Original languageEnglish (US)
Pages (from-to)340-347
Number of pages8
JournalCellular Immunology
Volume87
Issue number2
DOIs
StatePublished - Sep 1984

ASJC Scopus subject areas

  • Immunology

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    Goldschmidt-Clermont, P., Petrini, M., Khansari, N., & Fudenberg, H. H. (1984). The role of PNP enzyme in autologous rosette-forming cells. Cellular Immunology, 87(2), 340-347. https://doi.org/10.1016/0008-8749(84)90003-0