The role of pannexin 1 in the purinergic regulation of synaptic transmission in mouse motor synapses

A. S. Miteva, A. E. Gaydukovl, Valery I Shestopalov, O. P. Balezina

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The role of pannexin 1 in the release to the extracellular space of ATP/adenosine modulating the acetyicholine (ACh) secretion was studied in mouse diaphragm motor synapses. Using neuromuscular preparations obtained from wild-Type and pannexin-l knock-out mice, the miniature endplate potentials (MEPPs) and evoked endplate potentials (EPPs) were recorded in combination with pharmacological modulation of P2-Type ATP receptors and A1-Type adenosine receptors. Selective inhibition of A1 receptors with DPCPX or P2 receptors with PPADS increased quantal content of EPPs in wild-Type mice. MRS 2211, selective antagonist of P2Y13 receptors, produced the same effect. Activationof receptors A1 or P2Y13 by their agonists (2-CADO and IDP, respectively) decreased the EPP quantal content. It means that the activity of endogenous ATP and adenosine is synergistic and directed to depression of the ACh release. ARL67156, an inhibitor of synaptic ecto-ATPases, which blocks the hydrolysis of ATP to adenosine and increases the level of ATP in the synaptic cleft, prolonged EPPs without changing their quantal content. In pannexin-l knock-out mice there were no changes in the EPP quantal content and in other parameters of synaptic transmission as compared to wild-Type mice. However, down regulation of purinergic effects with antagonists of A1 or P2 receptors (DPCPX, PPADS, MRS 2211) did not change EPP quantal content and any other parameters of spontaneous or evoked ACh release in all cases. ARL67156 did not alter the temporal parameters of EPPs, either. Nevertheless, 2-CADO, an agonist of A1 receptors, decreased the EPP quantal content, while the agonist of P2Y13 receptors decreased the MEPP amplitude. Thus, in mice lacking pannexin 1, procedures revealing the presence and regulatory activity of synaptic ATP/adenosine did not change the parameters of synaptic transmission. The obtained data substantiate a mandatory role of pannexin 1 in the purinergic regulation of motor synapse activity by endogenous ATP/adenosine.

Original languageEnglish (US)
Pages (from-to)48-57
Number of pages10
JournalBiologicheskie Membrany
Volume34
Issue number5
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

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Synaptic Transmission
Synapses
Adenosine
Adenosine Triphosphate
Knockout Mice
Purinergic Agents
Inosine Diphosphate
Purinergic P2 Receptors
Adenosine A1 Receptors
Extracellular Space
Diaphragm
Evoked Potentials
Motor Activity
Hydrolysis
Down-Regulation
Pharmacology
pyridoxal phosphate-6-azophenyl-2',4'-disulfonic acid
1,3-dipropyl-8-cyclopentylxanthine
MRS 2211

Keywords

  • A1-Type adenosine receptors
  • Adenosine
  • AT?
  • P2Y13 purinergic receptors
  • Pannexin 1

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

The role of pannexin 1 in the purinergic regulation of synaptic transmission in mouse motor synapses. / Miteva, A. S.; Gaydukovl, A. E.; Shestopalov, Valery I; Balezina, O. P.

In: Biologicheskie Membrany, Vol. 34, No. 5, 01.01.2017, p. 48-57.

Research output: Contribution to journalArticle

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