The role of pannexin 1 in the purinergic regulation of synaptic transmission in mouse motor synapses

A. S. Miteva, A. E. Gaydukov, Valery I Shestopalov, O. P. Balezina

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The role of pannexin 1 in the release to the extracellular space of ATP/adenosine modulating the acetylcholine (ACh) secretion was studied in mouse diaphragm motor synapses. Using neuromuscular preparations obtained from wild-type and pannexin-1 knockout mice, the miniature endplate potential (MEPPs) and evoked endplate potentials (EPPs) were recorded in combination with pharmacological modulation of P2-type ATP receptors and A1-type adenosine receptors. Selective inhibition of A1 receptors with DPCPX or P2 receptors with PPADS increased quantal content of EPPs in wild-type mice. MRS 2211, selective antagonist of P2Y13 receptors, produced the same effect. Activation of receptors A1 or P2Y13 by their agonists (2-CADO and IDP, respectively) decreased the EPP quantal content. It means that the activity of endogenous ATP and adenosine is synergistic and directed to depression of the ACh release. ARL67156, an inhibitor of synaptic ecto-ATPases, which blocks the hydrolysis of ATP to adenosine and increases the level of ATP in the synaptic cleft, prolonged EPPs without changing their quantal content. In pannexin-1 knockout mice there were no changes in the EPP quantal content and in other parameters of synaptic transmission as compared to wildtype mice. However, downregulation of purinergic effects with antagonists of A1 or P2 receptors (DPCPX, PPADS, MRS 2211) did not change EPP quantal content and any other parameters of spontaneous or evoked ACh release in all cases. ARL67156 did not alter the temporal parameters of EPPs, either. Nevertheless, 2-CADO, the A1-type receptor agonist, decreased the EPP quantal content, while the agonist of P2Y13 receptors decreased the MEPP amplitude. Thus, in mice lacking pannexin 1, procedures revealing the presence and regulatory activity of synaptic ATP/adenosine did not change the parameters of synaptic transmission. The obtained data substantiate a mandatory role of pannexin 1 in the purinergic regulation of motor synapse activity by endogenous ATP/adenosine.

Original languageEnglish (US)
Pages (from-to)311-320
Number of pages10
JournalBiochemistry (Moscow) Supplement Series A: Membrane and Cell Biology
Volume11
Issue number4
DOIs
StatePublished - Oct 1 2017
Externally publishedYes

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Synaptic Transmission
Synapses
Adenosine
Adenosine Triphosphate
Acetylcholine
Bioelectric potentials
Knockout Mice
Purinergic Agents
Inosine Diphosphate
Purinergic P2 Receptors
Adenosine A1 Receptors
Extracellular Space
Diaphragms
Diaphragm
Evoked Potentials
Hydrolysis
Motor Activity
Down-Regulation
Chemical activation
Modulation

Keywords

  • A1-type adenosine receptors
  • adenosine
  • ATP
  • P2Y purinergic receptors
  • pannexin 1

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

The role of pannexin 1 in the purinergic regulation of synaptic transmission in mouse motor synapses. / Miteva, A. S.; Gaydukov, A. E.; Shestopalov, Valery I; Balezina, O. P.

In: Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology, Vol. 11, No. 4, 01.10.2017, p. 311-320.

Research output: Contribution to journalArticle

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