The role of pannexin 1 hemichannels in ATP release and cell-cell communication in mouse taste buds

Yi Jen Huang; Yutaka Maruyama; Gennady Dvoryanchikov; Elizabeth Pereira; Nirupa Chaudhari; Stephen D. Roper

doi:10.1073/pnas.0611280104

The role of pannexin 1 hemichannels in ATP release and cell-cell communication in mouse taste buds

Yi Jen Huang, Yutaka Maruyama, Gennady Dvoryanchikov, Elizabeth Pereira, Nirupa Chaudhari, Stephen D. Roper

Physiology & Biophysics

Research output: Contribution to journal › Article › peer-review

427 Scopus citations

Abstract

ATP has been shown to be a taste bud afferent transmitter, but the cells responsible for, and the mechanism of, its release have not been identified. Using CHO cells expressing high-affinity neurotransmitter receptors as biosensors, we show that gustatory stimuli cause receptor cells to secrete ATP through pannexin 1 hemichannels in mouse taste buds. ATP further stimulates other taste cells to release a second transmitter, serotonin. These results provide a mechanism to link intracellular Ca²⁺ release during taste transduction to secretion of afferent transmitter, ATP, from receptor cells. They also indicate a route for cell-cell communication and signal processing within the taste bud.

Original language	English (US)
Pages (from-to)	6436-6441
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	104
Issue number	15
DOIs	https://doi.org/10.1073/pnas.0611280104
State	Published - Apr 10 2007

Keywords

Afferent
Gustation
Serotonin
Synapses

ASJC Scopus subject areas

Genetics
General

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10.1073/pnas.0611280104

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The role of pannexin 1 hemichannels in ATP release and cell-cell communication in mouse taste buds. / Huang, Yi Jen; Maruyama, Yutaka; Dvoryanchikov, Gennady; Pereira, Elizabeth; Chaudhari, Nirupa ; Roper, Stephen D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 15, 10.04.2007, p. 6436-6441.

Research output: Contribution to journal › Article › peer-review

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