The role of pannexin 1 hemichannels in ATP release and cell-cell communication in mouse taste buds

Yi Jen Huang, Yutaka Maruyama, Gennady Dvoryanchikov, Elizabeth Pereira, Nirupa Chaudhari, Stephen D. Roper

Research output: Contribution to journalArticle

411 Scopus citations

Abstract

ATP has been shown to be a taste bud afferent transmitter, but the cells responsible for, and the mechanism of, its release have not been identified. Using CHO cells expressing high-affinity neurotransmitter receptors as biosensors, we show that gustatory stimuli cause receptor cells to secrete ATP through pannexin 1 hemichannels in mouse taste buds. ATP further stimulates other taste cells to release a second transmitter, serotonin. These results provide a mechanism to link intracellular Ca2+ release during taste transduction to secretion of afferent transmitter, ATP, from receptor cells. They also indicate a route for cell-cell communication and signal processing within the taste bud.

Original languageEnglish (US)
Pages (from-to)6436-6441
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number15
DOIs
StatePublished - Apr 10 2007

Keywords

  • Afferent
  • Gustation
  • Serotonin
  • Synapses

ASJC Scopus subject areas

  • Genetics
  • General

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